Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, I.R, Iran; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Placenta. 2021 Mar;106:49-57. doi: 10.1016/j.placenta.2021.02.008. Epub 2021 Feb 23.
Gestational diabetes mellitus (GDM) is a metabolic syndrome among pregnant mothers that increases the risk of developing growth disorders in the fetus and the placenta. Adiponectin is an adipokine, which plays a central role in the regulation of glucose and lipid metabolism, energy homeostasis, and insulin resistance in various tissues. Quercetin is a natural flavonoid with beneficial effects in the diabetic animal model, but data related to its effect on histological change and adiponectin system in the placenta of GDM are limited. In the current study, some histological changes and expression of adiponectin and its two receptors in the placenta of rats with GDM were investigated.
This study was carried out on placentas from the rodent model. To induce GDM, female rats were treated with a single dose of STZ. Placenta tissue was harvested and stained by PAS method. Protein and mRNA levels of adiponectin and its two receptors were assessed by immunohistochemistry and Real time PCR analysis, respectively.
The results showed the increased number of glycogen cells and thickness of the labyrinth interhemal membrane (LIM) in the embryonic part of the placenta in diabetic rats, while the use of quercetin significantly prevented their increase in diabetic rats. Treatment of the diabetic group with quercetin caused significantly increased adiponectin expression and decreased its receptors.The immunohistochemical study revealed the expression of AdipoR2 in the cytoplasm of syncytiotrophoblast and cytotrophoblast cells.
The results indicated that quercetin in pregnant diabetic rats could attenuate the histological abnormalities and improved adiponectin system dysregulation in the placenta.
妊娠糖尿病（GDM）是孕妇中的一种代谢综合征，会增加胎儿和胎盘生长障碍的风险。脂联素是一种脂肪因子，在调节葡萄糖和脂质代谢、能量平衡以及各种组织中的胰岛素抵抗方面发挥着核心作用。槲皮素是一种天然类黄酮，在糖尿病动物模型中具有有益作用，但关于其对 GDM 胎盘组织学变化和脂联素系统影响的数据有限。在本研究中，研究了 GDM 大鼠胎盘的一些组织学变化以及脂联素及其两种受体的表达。
本研究在啮齿动物模型的胎盘上进行。为了诱导 GDM，雌性大鼠单次给予 STZ 处理。通过 PAS 方法对胎盘组织进行染色。通过免疫组织化学和实时 PCR 分析分别评估脂联素及其两种受体的蛋白和 mRNA 水平。
结果表明，糖尿病大鼠胚胎部分胎盘的糖原细胞数量增加，细胞间血窦膜（LIM）增厚，而槲皮素的使用可显著防止其在糖尿病大鼠中的增加。糖尿病组用槲皮素处理后，脂联素表达显著增加，其受体减少。免疫组织化学研究显示 AdipoR2 在合体滋养细胞和细胞滋养细胞的细胞质中表达。
结果表明，槲皮素可减轻妊娠糖尿病大鼠的组织学异常，并改善胎盘的脂联素系统失调。
