V H Vishaka, Saxena Manav, R Geetha Balakrishna, Latiyan Sachin, Jain Shilpee
Center for Nano and Material Science, Jain University Jakkasandra Post Bangalore Rural-562112 India
Metallurgical and Materials Engineering, National Institute of Technology Tiruchirapalli-620015 India.
RSC Adv. 2019 Sep 2;9(47):27439-27448. doi: 10.1039/c9ra05256a. eCollection 2019 Aug 29.
The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells . Significantly trace levels of Fe ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe. As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe.
一种生物相容性荧光探针的强大特性得以展现，即便在真实人类血液样本和细胞中被醋酸盐反复淬灭（可逆性）后，它仍能检测到铁。能够检测到高达8.2 nM的显著痕量铁离子，不受其他各种金属离子存在的影响。所获结果通过原子吸收光谱法（AAS）和电感耦合等离子体发射光谱法（ICP - OES）进行了验证。还制备了一种便携式测试条，用于现场快速检测铁。由于铁是细胞中普遍存在的金属且在生物过程中发挥着重要作用，使用这种探针在细胞中对铁进行成像，是生物传感领域的一项重大进展。细胞毒性研究也证明了该探针的无毒性质。
