Kim Kang Hyeon, Wee Kyung Wook, Kim CheonJung, Hur Don, Lee Jeong Hoon, Yoo Yong Kyoung
Department of Electrical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul, 01897 South Korea.
Department of Electonic Engineering, Catholic Kwandong University, 24, Beomil-ro 579beon gil, Gangneung-si, Gangwond-do 25601 South Korea.
Biomed Eng Lett. 2022 Feb 8;12(2):197-203. doi: 10.1007/s13534-022-00219-x. eCollection 2022 May.
Field effect transistor (FET) biosensor is based on metal oxide field effect transistor that is gated by changes in the surface charges induced the reaction of biomolecules. In most cases of FET biosensor, FET biosensor is not being reused after the reaction; therefore, it is an important concept of investigate the biosensor with simplicity, cheap and reusability. However, the conventional cardiac troponin I (cTnI) sensing technique is inadequate owing to its low sensitivity and high operational time and cost. In this study, we developed a rapid and low-cost, and disposable electrical sensor using an extended gate field-effect transistor (EGFET) to detect cTnI, as a key biomarker for myocardiac infarction. We first investigated pH sensing characteristics according to the pH level, which provided a logarithmically linear sensitivity in the pH sensing buffer solution of approximately 57.9 mV/pH. Subsequently, we prepared a cTnI sample and monitored the reaction between cTnI and cTnI antibodies through the changes in the drain current and transfer curves. Our results showed that the EGFET biosensor could successfully detect the cTnI levels as well as the pH with low-cost and rapid detection.
场效应晶体管（FET）生物传感器基于金属氧化物场效应晶体管，其通过生物分子反应诱导的表面电荷变化来进行门控。在大多数FET生物传感器的情况下，反应后该生物传感器无法重复使用；因此，研究具有简单、廉价和可重复使用性的生物传感器是一个重要概念。然而，传统的心肌肌钙蛋白I（cTnI）传感技术由于其低灵敏度、高操作时间和成本而存在不足。在本研究中，我们开发了一种快速、低成本的一次性电传感器，使用扩展栅场效应晶体管（EGFET）来检测cTnI，cTnI是心肌梗死的关键生物标志物。我们首先根据pH值研究了pH传感特性，在pH传感缓冲溶液中其提供了约57.9 mV/pH的对数线性灵敏度。随后，我们制备了cTnI样品，并通过漏极电流和转移曲线的变化监测cTnI与cTnI抗体之间的反应。我们的结果表明，EGFET生物传感器能够以低成本和快速检测成功检测cTnI水平以及pH值。
