He Rong-Yu, Tseng Hsin-Yi, Lee Hsia-An, Liu Yu-Ci, Koshevoy Igor O, Pan Sheng-Wei, Ho Mei-Lin
Department of Chemistry, Soochow University No. 70, LinShih Rd., Shih-Lin Taipei 11102 Taiwan
Department of Chemistry, University of Eastern Finland 80101 Joensuu Finland.
RSC Adv. 2019 Oct 10;9(56):32367-32374. doi: 10.1039/c9ra04278d.
In this study, optical microfluidic paper analytical devices (μPADs) for glucose detection from whole blood samples with a small sample volume (2 μL) have been developed on a single paper. In the proposed method, a mushroom-shaped analytical device contains a sample inlet zone and a detection zone. When blood is dripped onto the inlet region of a μPAD, the plasma diffuses to the detection region. The detection region is implanted with a metallic three-dimensional (3D) polymer hydrogel vehicle. The gel vehicle consists of a copper complex that responds to oxygen changes and glucose oxidase (GOx) immobilized inside the gel as a bioactivity preservative. The phosphorescence of the copper complex is enhanced by oxygen consumed by detection of glucose with a limit of detection (S/N = 3) of 0.44 mM, and the total analysis of the sample is completed within 1 min. The validity of the proposed research is demonstrated using control samples and real-world whole blood samples of glucose concentrations ranging from 3 to 200 mM, and the detection results are shown to be in agreement with those obtained using a glucometer. Attaining a simple device for analysing glucose in human whole blood without any pretreatment procedures and having a broad sensing range while consuming a small sample volume remain challenging; thus, our new analytical device is of great interest.
在本研究中，已在单张纸上开发出用于从小体积（2 μL）全血样本中检测葡萄糖的光学微流控纸分析装置（μPADs）。在所提出的方法中，蘑菇形分析装置包含一个样品入口区和一个检测区。当血液滴加到μPAD的入口区域时，血浆扩散到检测区域。检测区域植入了一种金属三维（3D）聚合物水凝胶载体。该凝胶载体由一种对氧气变化有响应的铜配合物和固定在凝胶内作为生物活性防腐剂的葡萄糖氧化酶（GOx）组成。通过葡萄糖检测所消耗的氧气增强了铜配合物的磷光，检测限（S/N = 3）为0.44 mM，且样品的总分析在1分钟内完成。使用葡萄糖浓度范围为3至200 mM的对照样品和实际全血样品证明了所提出研究的有效性，检测结果显示与使用血糖仪获得的结果一致。在不进行任何预处理程序的情况下获得一种用于分析人体全血中葡萄糖的简单装置，同时具有宽传感范围且消耗小体积样品，仍然具有挑战性；因此，我们的新型分析装置具有很大的吸引力。
