Department of Chemistry, Soochow University, No. 70, LinShih Rd, Shih-Lin, Taipei 11102, Taiwan.
Dalton Trans. 2018 Jun 25;47(25):8346-8355. doi: 10.1039/c8dt00500a.
In this work, we present a facile preparation of a paper-based glucose assay for rapid, sensitive, and quantitative measurement of glucose in blood plasma and urine. Two copper phosphorescent complexes [Cu(2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline)(2,6-dimethylphenylisocyanide)2][B(C6H3(CF3)2)4] (Cu1) and [Cu(2,9-dimethyl-1,10-phenanthroline)(2,6-dimethylphenylisocyanide)2][B(C6H3(CF3)2)4] (Cu2) and a new silver congener [Ag(P3)CNAg(P3)][B(C6H3(CF3)2)4] (Ag3) (P3 = PPh2C6H4-PPh-C6H4PPh2 [bis(o-diphenylphosphinophenyl)phenylphosphine]) have been synthesized and their oxygen sensing abilities were investigated. The dimetallic phosphine-based Ag3 complex, having a high oxygen sensing ability, was employed as an efficient signal transducer in enzymatic reactions to recognize blood plasma glucose and urine glucose, which provided a wide linear response for a concentration range between 1.0 and 35 mM and a rapid response, with a limit of detection (LOD) of 0.09 mM for glucose. In practical application, this Ag3 paper-based device offers great analytical reliability and accuracy upon monitoring glucose concentrations in blood plasma.
在这项工作中，我们提出了一种简便的制备基于纸张的葡萄糖分析方法，用于快速、灵敏、定量测量血浆和尿液中的葡萄糖。我们合成了两种铜磷光配合物 [Cu(2,9-二甲基-4,7-二苯基-1,10-菲咯啉)(2,6-二甲基苯基异氰化物)2][B(C6H3(CF3)2)4] (Cu1) 和 [Cu(2,9-二甲基-1,10-菲咯啉)(2,6-二甲基苯基异氰化物)2][B(C6H3(CF3)2)4] (Cu2)，以及一种新的银同系物 [Ag(P3)CNAg(P3)][B(C6H3(CF3)2)4] (Ag3)（P3 = PPh2C6H4-PPh-C6H4PPh2 [双(o-二苯基膦基苯基)苯基膦]），并研究了它们的氧传感能力。具有高氧传感能力的二价磷基银 3 配合物被用作酶反应中的有效信号转导体，以识别血浆葡萄糖和尿液葡萄糖，该方法为 1.0 至 35 mM 的浓度范围内提供了宽线性响应和快速响应，葡萄糖的检测限 (LOD) 为 0.09 mM。在实际应用中，这种 Ag3 基于纸张的设备在监测血浆葡萄糖浓度时提供了出色的分析可靠性和准确性。
