Cox R A, Pope R M
Infect Immun. 1987 May;55(5):1058-62. doi: 10.1128/iai.55.5.1058-1062.1987.
Lymphocyte transformation (LT) responses to coccidioidin (CDN) and spherulin were suppressed in 11 (73%) of 15 patients with active coccidioidomycosis when their mononuclear cells were assayed in autologous serum as compared to serum from healthy, CDN skin test-positive subjects. Suppressed LT responses were specific for Coccidioides immitis antigens in 7 (64%) of the 11 patients. Immunoaffinity chromatography of patient sera with Staphylococcus protein A adsorbed the suppressor component(s) and thereby established that suppression was attributed to immunoglobulin G, either alone or complexed with antigen. The possibility that suppression was mediated by immune complexes was examined by adding complexes formed in vivo or in vitro to mononuclear cell cultures of healthy CDN-reactive persons before LT assays. Although complexes prepared in this manner were reactive in an enzyme-linked immunosorbent assay designed to detect Coccidioides antigen-specific immune complexes, no suppression of LT responses was observed. We conclude that serum-mediated suppression of LT responses in coccidioidomycosis is attributed to monomeric and not immune-complexed immunoglobulin G antibody.
在15例活动性球孢子菌病患者中，当用自体血清而非健康的球孢子菌素（CDN）皮肤试验阳性受试者的血清检测其单核细胞时，11例（73%）患者对球孢子菌素（CDN）和球孢子菌蛋白的淋巴细胞转化（LT）反应受到抑制。11例患者中有7例（64%）的LT反应抑制对粗球孢子菌抗原具有特异性。用葡萄球菌蛋白A对患者血清进行免疫亲和层析吸附了抑制成分，从而确定抑制作用归因于免疫球蛋白G，其可以单独存在或与抗原复合。在LT检测之前，通过将体内或体外形成的复合物添加到健康的对CDN有反应者的单核细胞培养物中，来检验抑制作用是否由免疫复合物介导。尽管以这种方式制备的复合物在旨在检测粗球孢子菌抗原特异性免疫复合物的酶联免疫吸附试验中有反应，但未观察到LT反应受到抑制。我们得出结论，球孢子菌病中血清介导的LT反应抑制归因于单体免疫球蛋白G抗体，而非免疫复合物形式的免疫球蛋白G抗体。
