Lin Daobin, Ding Yi, Cheng Yabo, Chen Yubin, Tang Yunting, Wu Xiaowen, Cheng Yawei
Department of Cardiology, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570293, China.
Department of Disease Prevention, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570293, China.
Evid Based Complement Alternat Med. 2022 Apr 28;2022:4897463. doi: 10.1155/2022/4897463. eCollection 2022.
Nonalcoholic fatty liver disease (NAFLD) is currently the major cause of chronic liver disease globally. To observe the sedative effect of extract (MFE) on NAFLD and the potential molecular mechanism, a high-fat diet (HFD) was used to induce NAFLD in rats for 8 weeks. Rats were orally given MFE (1.7 g/kg, 2.5 g/kg, and 3.3 g/kg) every day. Serum and liver biochemical indexes were detected. 16S rDNA sequencing was performed to test the changes in the gut microbiota. Mass spectrometry was used to analyze the changes in blood and liver metabolites and to perform a joint analysis of differential flora and differential metabolites. The results showed that MFE alleviated liver injury and decreased hepatic lipids content. ELISA analysis certificated that MFE reduced inflammation levels in rats fed with HFD. Compared to HFD rats with a normal diet, MFE significantly changed the overall structure of the intestinal flora and the composition of the intestinal microbes destroyed by HFD. In addition, MFE changes the metabolic levels of lipids and proteins in HFD rats. In conclusion, MFE effectively treated NAFLD and significantly improved the overall structure and intestinal microbial composition of the intestinal microbiota. The abundance of and increased significantly in the partridge tea treatment group.
非酒精性脂肪性肝病（NAFLD）目前是全球慢性肝病的主要原因。为观察提取物（MFE）对NAFLD的镇静作用及潜在分子机制，采用高脂饮食（HFD）诱导大鼠发生NAFLD 8周。大鼠每天口服给予MFE（1.7 g/kg、2.5 g/kg和3.3 g/kg）。检测血清和肝脏生化指标。进行16S rDNA测序以检测肠道微生物群的变化。采用质谱法分析血液和肝脏代谢产物的变化，并对差异菌群和差异代谢产物进行联合分析。结果表明，MFE减轻了肝损伤并降低了肝脏脂质含量。ELISA分析证实，MFE降低了高脂饮食喂养大鼠的炎症水平。与正常饮食的HFD大鼠相比，MFE显著改变了肠道菌群的整体结构以及被HFD破坏的肠道微生物组成。此外，MFE改变了HFD大鼠的脂质和蛋白质代谢水平。总之，MFE有效治疗了NAFLD，并显著改善了肠道微生物群的整体结构和肠道微生物组成。鹧鸪茶治疗组中[具体菌种名称未给出]和[具体菌种名称未给出]的丰度显著增加。
