Kamiya Koki, Osaki Toshihisa, Takeuchi Shoji
Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology 3-2-1 Sakado, Takatsu-ku Kawasaki Kanagawa 213-0012 Japan.
Division of Molecular Science, Graduate School of Science and Technology, Gunma University 1-5-1 Tenjin-cho Kiryu Gunma 376-8515 Japan.
RSC Adv. 2019 Sep 24;9(52):30071-30075. doi: 10.1039/c9ra04622d. eCollection 2019 Sep 23.
Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inner leaflet. The lipid asymmetricities both in the intracellular vesicle membrane and the plasma membrane contribute to synaptic transmission functions. In this study, we developed a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles (of diameter 200-1000 nm) (asymmetric vesicles-in-a-vesicle), emulating lipid components in the plasma membrane and intracellular vesicle membrane of eukaryotic cells, using microfluidic technology. We successfully constructed an artificial exocytosis system using the asymmetric vesicles-in-a-vesicle system. This asymmetric vesicles-in-a-vesicle system will be helpful in understanding the mechanisms of vesicle transport, such as neurotransmission and exocytosis.
细胞内囊泡中的脂质分布与真核细胞的质膜中的脂质分布不同。细胞内囊泡中的脂质成分在外层小叶由磷脂酰丝氨酸和磷脂酰乙醇胺组成，在内层小叶由磷脂酰胆碱和鞘磷脂组成。细胞内囊泡膜和质膜中的脂质不对称性都有助于突触传递功能。在本研究中，我们利用微流控技术开发了一种细胞大小的不对称脂质囊泡系统，该系统包含小尺寸的不对称脂质囊泡（直径200 - 1000 nm）（囊泡内的不对称囊泡），模拟真核细胞质膜和细胞内囊泡膜中的脂质成分。我们利用囊泡内的不对称囊泡系统成功构建了人工胞吐系统。这种囊泡内的不对称囊泡系统将有助于理解囊泡运输机制，如神经传递和胞吐作用。
