Division of Applied Life Science (BK21Four), Gyeongsang National University, Jinju, Korea.
National Institute of Animal Science, Rural Development Administration, Cheonan, Korea.
PLoS One. 2021 Jan 29;16(1):e0246290. doi: 10.1371/journal.pone.0246290. eCollection 2021.
Studies that screen for metabolites produced in ruminants are actively underway. We aimed to evaluate the metabolic profiles of five biofluids (ruminal fluid, serum, milk, urine, and feces) in dairy cow by using proton nuclear magnetic resonance (1H-NMR) and provide a list of metabolites in each biofluid for the benefit of future research. We analyzed the metabolites in five biofluids from lactating cows using proton nuclear magnetic resonance imaging; 96, 73, 88, 118, and 128 metabolites were identified in the five biofluids, respectively. In addition, 8, 6, 9, and 17 metabolites were unique to ruminal fluid, serum, milk, and urine, respectively. The metabolites present at high concentrations were: acetate, propionate, and butyrate in ruminal fluid; lactate, glucose, and acetate in serum; and lactose, guanidoacetate, and glucitol in milk. In addition, the following metabolites were present at high concentrations: hippurate, urea, and trimethylamine N-oxide in urine and acetate, propionate, and butyrate in feces. The score plots of the principal component analysis did not show clear distinctions among the five biofluid samples. The purpose of this study was to verify the ability of our metabolomics approaches to identify metabolites in the biofluids of dairy cows.
目前正在积极开展针对反刍动物产生的代谢产物进行筛选的研究。我们旨在通过使用质子核磁共振（1H-NMR）来评估奶牛的五种生物流体（瘤胃液、血清、牛奶、尿液和粪便）的代谢谱，并为未来的研究提供每种生物流体中的代谢物列表。我们使用质子核磁共振成像分析了泌乳奶牛的五种生物流体中的代谢物；在五种生物流体中分别鉴定出 96、73、88、118 和 128 种代谢物。此外，瘤胃液、血清、牛奶和尿液中分别有 8、6、9 和 17 种代谢物是独特的。高浓度存在的代谢物为：瘤胃液中的乙酸盐、丙酸盐和丁酸盐；血清中的乳酸盐、葡萄糖和乙酸盐；牛奶中的乳糖、胍基乙酸盐和山梨糖醇。此外，尿中的马尿酸、尿素和三甲胺 N-氧化物以及粪便中的乙酸盐、丙酸盐和丁酸盐也以高浓度存在。主成分分析的得分图没有显示五种生物流体样本之间的明显区别。本研究的目的是验证我们的代谢组学方法识别奶牛生物流体中代谢物的能力。
