Gong Lei, Geng Hongjuan, Zhang Xi, Gao Ping
Department of Esophageal Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital Tianjin 300070 PR China.
Department of Stomatology Tianjin Hospital, 406 Jiefang South Road, Hexi District Tianjin 300211 PR China.
RSC Adv. 2019 Aug 21;9(45):26276-26282. doi: 10.1039/c9ra05127a. eCollection 2019 Aug 19.
Peri-implantitis is a plaque-initiating infectious disease that can be prevented by interfering with the initial bacterial attachment. At present, surface modification of implants using antimicrobial peptides can interfere with the adhesion of streptococci. In this study, the structure and function of chimeric peptides were compared to get a strategy to modify a Ti surface. Compared to the antimicrobial activity with a fragment of hBD-3, the bifunctional and multifunctional chimeric peptides retain their antimicrobial function. All peptides showed antimicrobial activity against streptococcus in biofilm and planktonic conditions. The results demonstrate significant improvement in reducing bacterial colonization onto titanium surfaces. According to the results of structure analysis, the antimicrobial activity of tyrosine in hBD3-3 was stronger than that of the alpha helix in bifunctional or multifunctional chimeric peptides. Rigid connections were proved to avoid functional domain changes due to the interaction of charges. These results indicated that the endogenous peptide fragments modifying the Ti surface could provide an environmentally friendly approach to reduce or prevent the occurrence of peri-implant diseases.
种植体周围炎是一种由菌斑引发的感染性疾病，可通过干扰细菌的初始附着来预防。目前，使用抗菌肽对种植体进行表面修饰可干扰链球菌的黏附。在本研究中，对嵌合肽的结构和功能进行了比较，以获得一种修饰钛表面的策略。与hBD-3片段的抗菌活性相比，双功能和多功能嵌合肽保留了它们的抗菌功能。所有肽在生物膜和浮游状态下均对链球菌表现出抗菌活性。结果表明，在减少细菌在钛表面的定植方面有显著改善。根据结构分析结果，hBD3-3中酪氨酸的抗菌活性强于双功能或多功能嵌合肽中的α螺旋。事实证明，刚性连接可避免因电荷相互作用而导致功能域发生变化。这些结果表明，修饰钛表面的内源性肽片段可为减少或预防种植体周围疾病的发生提供一种环境友好的方法。
