Parvez Mohammad K, Al-Dosari Mohammed S, Ahmed Sarfaraz, Noman Omar M, Al-Rehaily Adnan J, Nur-E-Alam Mohammad
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Saudi J Biol Sci. 2022 Apr;29(4):3062-3068. doi: 10.1016/j.sjbs.2022.01.041. Epub 2022 Jan 22.
The genus constitutes cold-adapted plant spp., of these some are traditionally used in folk medicine against inflammation or fungal infections without scientific validations. Here, we report the biological activities of total ethanol-extract (CF-EtOH) and its hexane (CF-Hex), ethyl acetate (CF-EtOA), butanol (CF-ButOH), and aqueous (CF-Aqua) fractions. Our DPPH and ABTS radical-scavenging assays showed CF-EtOH, CF-ButOH and CF-Aqua with maximal, CF-EtOA with moderate, and CF-Hex with mild anti-oxidant activities. When tested on human cancer cell lines, high cytotoxicity was demonstrated by CF-EtOH (IC: 42.45 μg/ml) and CF-Aqua (IC: 46.37 μg/ml) on HepG2, followed by CF-Hex (IC: 63.24 μg/ml) and CF-ButOH (IC: 65.32 μg/ml) on MCF7 cells. The human primary cell line (HUVEC) had comparatively lower cytotoxicity for the tested samples. Moreover, when assessed for anti-microbial efficacy, CF-ButOH and CF-Aqua exhibited the strongest activity (MIC: 156.25 μg/ml) against , and . Further, while the developed RP-HPTLC identified the bioactive flavonoid luteolin-7--glucoside (17.58 mg/g), GS/MS analysis revealed sixteen compounds in extract. In conclusion, we for the first time show the promising anti-oxidative, anti-cell proliferative and anti-microbial efficacies of . This warrants further phytochemical and bio-efficacy studies towards isolations and identifications of active principles.
该属包含适应寒冷环境的植物物种，其中一些传统上用于民间医学治疗炎症或真菌感染，但缺乏科学验证。在此，我们报告了[植物名称]总乙醇提取物（CF-EtOH）及其己烷（CF-Hex）、乙酸乙酯（CF-EtOA）、丁醇（CF-ButOH）和水相（CF-Aqua）馏分的生物活性。我们的DPPH和ABTS自由基清除试验表明，CF-EtOH、CF-ButOH和CF-Aqua具有最大抗氧化活性，CF-EtOA具有中等抗氧化活性，CF-Hex具有轻度抗氧化活性。在人癌细胞系上进行测试时，CF-EtOH（IC：42.45μg/ml）和CF-Aqua（IC：46.37μg/ml）对HepG2细胞表现出高细胞毒性，其次是CF-Hex（IC：63.24μg/ml）和CF-ButOH（IC：65.32μg/ml）对MCF7细胞的毒性。人原代细胞系（HUVEC）对测试样品的细胞毒性相对较低。此外，在评估抗菌效果时，CF-ButOH和CF-Aqua对[细菌名称]表现出最强的活性（MIC：156.25μg/ml）。此外，虽然所开发的RP-HPTLC鉴定出生物活性黄酮木犀草素-7-O-葡萄糖苷（17.58mg/g），但GS/MS分析揭示了[植物名称]提取物中的16种化合物。总之，我们首次展示了[植物名称]具有有前景的抗氧化、抗细胞增殖和抗菌功效。这需要进一步进行植物化学和生物功效研究，以分离和鉴定活性成分。
