Riaz Sana, Javed Muhammad Arslan, Nawaz Iqra, Javed Tariq
Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan.
Departments of Microbiology and Molecular Genetics, the Women University, Multan, Pakistan.
Saudi J Biol Sci. 2022 Apr;29(4):2421-2431. doi: 10.1016/j.sjbs.2021.12.015. Epub 2021 Dec 13.
In this study bioassay-guided screening of Tecomella undulate was performed for its cytotoxic, antimutagenic and anticancer potential. The ariel parts were extracted on a polarity basis (methanol, dichloromethane and hexane). The in vivo toxicity was assessed on Caenorhabditis elegans, and its locomotion was affected by Tecomella undulata hexane (TUAH) the most. Ames test for antimutagenicity showed Tecomella undulata methanol (TUAM) exhibited against mutagen 2AA showed inhibition of 71.03% and 26.32% 2AA in TA98 while in in vitro MTT assay on carcinoma cell lines TUAM showed 68.1% cytotoxicity. Moreover, In resazurin assay on fibroblast cells African green monkey kidney VERO and on the panel of carcinoma cell lines, the most effective extract was TUAM on liver HepG-2 with CC value 117.37 ± 4.73 µg/ml followed by on lungs A549 with 142.01 ± 5.3. Furthermore, for the bioassay-guided screening, the selectivity index was calculated for TUAM CC ratio on HepG-2 and VERO which showed a decent 2.77 score. After column chromatography, the fraction TU-63 should remarkable cytotoxic effect in dose-response manner assay as (Hep-G2) CC value 11. 67 ± 1.37 µg/ml followed by (A549) CC value 17.23 ± 0.58 µg/ml. For qualitative analysis of anticancer potential LC-ESI-MS/MS the potential phytochemicals were identified. In silico molecular modelling against selected carcinogenic proteins. The results suggest Tecomella undulate the substantial anticancer potential which supports potential natural anticancer therapeutic drug candidate development for combating cancer.
在本研究中，对小花罗布麻进行了生物测定指导的筛选，以评估其细胞毒性、抗诱变和抗癌潜力。地上部分按极性进行提取（甲醇、二氯甲烷和己烷）。对秀丽隐杆线虫进行了体内毒性评估，其运动受小花罗布麻己烷提取物（TUAH）影响最大。抗诱变的艾姆斯试验表明，小花罗布麻甲醇提取物（TUAM）对诱变剂2AA的抑制率在TA98中为71.03%，在TA100中为26.32%；而在癌细胞系的体外MTT试验中，TUAM显示出68.1%的细胞毒性。此外，在对非洲绿猴肾VERO成纤维细胞和一组癌细胞系的刃天青试验中，最有效的提取物是TUAM，对肝癌HepG-2细胞的CC值为117.37±4.73μg/ml，其次是对肺癌A549细胞，CC值为142.01±5.3。此外，对于生物测定指导的筛选，计算了TUAM在HepG-2和VERO细胞上的CC比值的选择性指数，显示出不错的2.77分。柱色谱分离后，馏分TU-63在剂量反应试验中表现出显著的细胞毒性作用，对Hep-G2细胞的CC值为11.67±1.37μg/ml，对A549细胞的CC值为17.23±0.58μg/ml。通过LC-ESI-MS/MS对抗癌潜力进行定性分析，鉴定了潜在的植物化学物质。针对选定的致癌蛋白进行了计算机分子模拟。结果表明小花罗布麻具有显著的抗癌潜力，这为开发对抗癌症的潜在天然抗癌治疗药物候选物提供了支持。
