Yin Xiujuan, Fang Ziyuan, Fang Yan, Zhu Lin, Pang Jinwen, Liu Tianjun, Zhao Zhanjuan, Zhao Jianxi
School of Clinical Medicine, Hebei University, Baoding, China.
Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin, China.
Front Microbiol. 2022 Apr 22;13:876166. doi: 10.3389/fmicb.2022.876166. eCollection 2022.
Osteomyelitis is deep tissue inflammation caused by bacterial infection. If such an infection persists, it can lead to dissolution and necrosis of the bone tissue. As a result of the extensive use of antibiotics, drug-resistant bacteria are an increasingly common cause of osteomyelitis, limiting the treatment options available to surgeons. Photodynamic antibacterial chemotherapy has attracted increasing attention as a potential alternative treatment. Its advantages are a broad antibacterial spectrum, lack of drug resistance, and lack of toxic side effects. In this study, we explored the impact of the new photosensitizer LD4 in photodynamic antimicrobial chemotherapy (PACT), both alone and in combination with an antibiotic, on osteomyelitis. A rabbit tibial osteomyelitis model was employed and microbiological, histological, and radiological studies were performed. New Zealand white rabbits ( = 36) were randomly divided into a control group, antibiotic group, PACT group and PACT + antibiotic group for treatment. In microbiological analysis, a reduction in bacterial numbers of more than 99.9% was recorded in the PACT group and the PACT + antibiotic group 5 weeks after treatment ( < 0.01). In histological analysis, repair of the damaged bone tissue was observed in the PACT group, and bone repair in the PACT + antibiotic group was even more significant. In radiological analysis, the X-ray Norden score showed that the severity of bone tissue defects or destruction followed the pattern: PACT + antibiotic group < PACT group < antibiotic group < control group.
骨髓炎是由细菌感染引起的深部组织炎症。如果这种感染持续存在，可导致骨组织溶解和坏死。由于抗生素的广泛使用，耐药菌日益成为骨髓炎的常见病因，限制了外科医生的治疗选择。光动力抗菌化疗作为一种潜在的替代治疗方法，越来越受到关注。其优点是抗菌谱广、无耐药性且无毒性副作用。在本研究中，我们探讨了新型光敏剂LD4在光动力抗菌化疗（PACT）中单独使用以及与抗生素联合使用对骨髓炎的影响。采用兔胫骨骨髓炎模型，并进行了微生物学、组织学和放射学研究。将36只新西兰白兔随机分为对照组、抗生素组、PACT组和PACT+抗生素组进行治疗。微生物学分析显示，治疗5周后，PACT组和PACT+抗生素组细菌数量减少超过99.9%（P<0.01）。组织学分析观察到PACT组受损骨组织有修复，PACT+抗生素组的骨修复更显著。放射学分析中，X线诺登评分显示骨组织缺损或破坏的严重程度依次为：PACT+抗生素组<PACT组<抗生素组<对照组。
