Lu Yating, Guo Yue, Liang Xiao, Huang Huimin, Ling Xue, Su Zhiheng, Liang Yonghong
Pharmaceutical College, Guangxi Medical University, Nanning 530021, China.
Department of Pharmacy, The Fifth Affiliated Hospital of Guangxi Medical University, The First People's Hospital of Nanning, Nanning, China.
Anal Methods. 2022 May 27;14(20):1963-1972. doi: 10.1039/d2ay00492e.
Aristolochic acid I (AAI) is one of the nephrotoxic derivatives present in genera and . Although some detection strategies for monitoring AAI have been reported, the application of these methods is limited because they involve tedious preparation and require professional operation. In this work, bovine serum albumin (BSA) has been introduced as a reducing agent and stabilizing agent to synthesize gold nanoclusters with strong red fluorescence for the rapid and effective detection of AAI. Under excitation at 328 nm, the fluorescence intensity at the maximum emission wavelength of the bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) decreased with the addition of AAI, and the degree of quenching showed a linear relationship with the concentration of AAI from 0.1-12.8 μg mL. The obtained BSA-AuNCs were stable, and quenching in the presence of AAI could be achieved within 10 seconds. Here, we have focused on the application of these gold nanoclusters as an optical sensing material for AAI in rat urine samples, including a discussion on the detection mechanism. The detection result of the fluorescent probe was consistent with that of the HPLC method. In view of this reality, the reported protein-AuNCs sensing platform can serve as a convenient detection strategy in toxicological analyses.
马兜铃酸I（AAI）是存在于马兜铃属和细辛属植物中的肾毒性衍生物之一。尽管已经报道了一些监测AAI的检测策略，但这些方法的应用受到限制，因为它们涉及繁琐的样品制备且需要专业操作。在这项工作中，引入牛血清白蛋白（BSA）作为还原剂和稳定剂，合成具有强红色荧光的金纳米簇，用于快速有效地检测AAI。在328nm激发下，牛血清白蛋白稳定的金纳米簇（BSA-AuNCs）在最大发射波长处的荧光强度随AAI的加入而降低，猝灭程度与AAI浓度在0.1-12.8μg/mL范围内呈线性关系。所制备的BSA-AuNCs稳定性良好，在AAI存在下10秒内即可实现猝灭。在此，我们重点研究了这些金纳米簇作为AAI光学传感材料在大鼠尿液样本中的应用，包括对检测机制的讨论。荧光探针的检测结果与高效液相色谱法一致。鉴于此，所报道的蛋白质-AuNCs传感平台可作为毒理学分析中的一种便捷检测策略。
