用于选择性检测血清叶绿酸铜钠的双蛋白配体修饰金纳米簇

Dual protein ligand-modified gold nanoclusters for selective detection of serum sodium copper chlorophyllin.

作者信息

Li Xiangfei, Qiao Juan, Li Zhiwei, Qi Li

机构信息

Key Laboratory of Analytical Chemistry for Living Biosystems, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, China; College of Chemical and Phamaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.

Key Laboratory of Analytical Chemistry for Living Biosystems, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, China; School of Chemical Sciences, University of Chinese Academy of Sciences, No.19A Yuquanlu, Beijing 100049, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 15;243:118798. doi: 10.1016/j.saa.2020.118798. Epub 2020 Aug 5.

DOI:10.1016/j.saa.2020.118798

PMID:32854084

Abstract

Precisely tuning the emission wavelength of dual ligands co-functionalized gold nanoclusters (AuNCs) presents a bright prospect for highly selective drug detection. In this study, gold nanoclusters (AuNCs) with strong red fluorescence at 633 nm were synthesized using bovine serum albumin (BSA) and bromelain (Bro) as dual ligands for highly selective detection of sodium copper chlorophyllin (SCC). Interestingly, the auto-fluorescence interference of the dual ligands was effectively avoided due to the fluorescence emission red-shifts of the prepared BSA/Bro@AuNCs; this may be mainly due to the rigid structure of the proteins. The fluorescence of BSA/Bro@AuNCs was quenched after addition of SCC, which performed well and provided a good linear calibration curve in the 5.0-25.0 μM range (R = 0.999), and a detection limit as low as 0.5 μM. The proposed assay was further applied to the measurement of SCC in rat serums after an intravenous injection. The dual protein ligand-capped AuNCs showed great potential for drug analysis in actual biological samples.

摘要

精确调控双配体共功能化金纳米簇（AuNCs）的发射波长在高选择性药物检测方面展现出光明前景。在本研究中，以牛血清白蛋白（BSA）和菠萝蛋白酶（Bro）作为双配体合成了在633 nm处具有强红色荧光的金纳米簇（AuNCs），用于高选择性检测叶绿素铜钠（SCC）。有趣的是，由于所制备的BSA/Bro@AuNCs荧光发射红移，有效避免了双配体的自发荧光干扰；这可能主要归因于蛋白质的刚性结构。加入SCC后，BSA/Bro@AuNCs的荧光猝灭，其表现良好，在5.0 - 25.0 μM范围内提供了良好的线性校准曲线（R = 0.999），检测限低至0.5 μM。所提出的检测方法进一步应用于静脉注射后大鼠血清中SCC的测定。双蛋白配体封端的AuNCs在实际生物样品的药物分析中显示出巨大潜力。