Institute of Medical and Biological Engineering, School of Mechanical Engineering, University of Leeds, Leeds, UK.
School of Biomedical Engineering, University of Technology Sydney, Ultimo, New South Wales, Australia.
J Biomed Mater Res B Appl Biomater. 2022 Oct;110(10):2276-2289. doi: 10.1002/jbm.b.35076. Epub 2022 May 9.
Submicron-sized wear particles are generally accepted as a potential cause of aseptic loosening when produced in sufficient volumes. With the accelerating use of increasingly wear-resistant biomaterials, identifying such particles and evaluating their biological response is becoming more challenging. Highly sensitive wear particle isolation methods have been developed but these methods cannot isolate the complete spectrum of particle types present in individual tissue samples. Two established techniques were modified to create one novel method to isolate both high- and low-density materials from periprosthetic tissue samples. Ten total hip replacement and eight total knee replacement tissue samples were processed. All particle types were characterized using high resolution scanning electron microscopy. UHMWPE and a range of high-density materials were isolated from all tissue samples, including: polymethylmethacrylate, zirconium dioxide, titanium alloy, cobalt chromium alloy and stainless steel. This feasibility study demonstrates the coexistence of mixed particle types in periprosthetic tissues and provides researchers with high-resolution images of clinically relevant wear particles that could be used as a reference for future in vitro biological response studies.
当产生足够数量的亚微米级磨损颗粒时，人们普遍认为它们是无菌性松动的潜在原因。随着越来越多的耐磨生物材料的加速使用，识别这些颗粒并评估它们的生物学反应变得更加具有挑战性。已经开发出了高度敏感的磨损颗粒分离方法，但这些方法无法分离出单个组织样本中存在的完整颗粒类型谱。对两种已建立的技术进行了修改，创建了一种新方法，可从假体周围组织样本中分离出高密度和低密度材料。共处理了 10 个全髋关节置换和 8 个全膝关节置换组织样本。使用高分辨率扫描电子显微镜对所有颗粒类型进行了表征。从所有组织样本中均分离出了超高分子量聚乙烯和一系列高密度材料，包括：聚甲基丙烯酸甲酯、二氧化锆、钛合金、钴铬合金和不锈钢。这项可行性研究证明了假体周围组织中混合颗粒类型的共存，并为研究人员提供了具有临床相关性的磨损颗粒的高分辨率图像，这些图像可作为未来体外生物学反应研究的参考。
