Synthetic Biology Engineering Lab of Henan Province, College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Arch Microbiol. 2022 May 9;204(6):305. doi: 10.1007/s00203-022-02927-5.
A novel Gram-stain-negative, non-motile, short rod-shaped and aerobic bacterial strain, designated as CFH 74456, was isolated from sediment of a hot spring, Tengchong, Yunnan Province, south-western China. Growth occurred at 20-53 ºC (optimum 45 ºC), pH 7.0-9.0 (optimum pH 8.0) and up to 2.0% (w/v) NaCl (optimum 0-1.0%, w/v). The predominant respiratory quinone was ubiquinone 10 (Q-10). The major fatty acids (> 10%) were C ω6c (17.9%) and summed feature 8 (38.6%). The polar lipid profile of strain CFH 74456 was identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, three unidentified glycolipids and three unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CFH 74456 belongs to the genus Qipengyuania, and was most closely related to Qipengyuania sediminis CGMCC 1.12928 (95.7%). The draft genome size of the isolate was 2.29 Mb with G + C content of 68.5%. The amino acid identity, average nucleotide identity and the digital DNA-DNA hybridization values between strain CFH 74456 and the closest relatives ranged from 67.0 to 67.9%, 73.0 to 74.2% and 18.2-19.3%, respectively. On the basis of phenotypic, phylogenetic and genotypic analyses, it is concluded that strain CFH 74456 represents a new species of the genus Qipengyuania, for which the name Qipengyuania thermophila sp. nov. is proposed. The type strain is CFH 74456 (= KCTC 62921 = CCTCC AB 2018237).
一株新的革兰氏阴性、非运动、短杆状和需氧细菌菌株，被命名为 CFH 74456，从中国西南部云南省腾冲温泉的沉积物中分离得到。该菌株在 20-53℃（最佳温度 45℃）、pH 值 7.0-9.0（最佳 pH 值 8.0）和高达 2.0%（w/v）NaCl（最佳 0-1.0%，w/v）的条件下生长。主要的呼吸醌是泛醌 10（Q-10）。主要脂肪酸（>10%）为 C ω6c（17.9%）和总和特征 8（38.6%）。菌株 CFH 74456 的极性脂谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、神经酰胺糖脂、三种未鉴定的糖脂和三种未鉴定的极性脂组成。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CFH 74456 属于 Qipengyuania 属，与 Qipengyuania sediminis CGMCC 1.12928（95.7%）最为密切相关。该分离株的基因组大小约为 2.29 Mb，G+C 含量为 68.5%。菌株 CFH 74456 与最接近的亲缘关系之间的氨基酸同一性、平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 67.0-67.9%、73.0-74.2%和 18.2-19.3%。基于表型、系统发育和基因型分析，结论认为菌株 CFH 74456 代表 Qipengyuania 属的一个新种，建议将其命名为嗜热 Qipengyuania 新种。模式菌株为 CFH 74456（=KCTC 62921=CCTCC AB 2018237）。
