Key Laboratory of Analytical Science for Food Safety and Biology (MOE and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
Department of Gastrointestinal Surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, No. 134 Dongjie, Fuzhou 350001, People's Republic of China.
Anal Chem. 2022 May 24;94(20):7408-7416. doi: 10.1021/acs.analchem.2c01133. Epub 2022 May 9.
Functional photothermal nanomaterials have gained widespread attention in the field of precise cancer therapy and early disease diagnosis due to their unique photothermal conversion properties. However, the relatively narrow temperature response range and the outputable accuracy of commercial thermometers limit the accurate detection of biomarkers. Herein, we designed a liposome-embedded CuAgS amplification-based photothermal sensor for the accurate determination of cardiac troponin I (cTnI) in health monitoring and point-of-care testing (POCT). The combinable 3D-printing detecting device monitored and visualized target signal changes in the testing system under the excitation of near-infrared (NIR) light, which was recorded and evaluated for possible pathogenicity by a smartphone. Notably, we predicted the potentially efficient thermal conversion efficiency of CuAgS from the structure and charge density distribution, calculated by the first-principles and density functional theory (DFT), which provided a theoretical basis for the construction of novel photothermal materials, and the experimental results proved the correctness of the theoretical projections. Under optimal conditions, the photothermal immunoassay showed a dynamic linear range of 0.02-10 ng mL with a detection limit of 11.2 pg mL. This work instructively introduces promising theoretical research and provides new insights for the development of sensitive portable photothermal biosensors.
功能型光热纳米材料因其独特的光热转换性能，在精准癌症治疗和早期疾病诊断领域得到了广泛关注。然而，商业温度计较窄的温度响应范围和输出精度限制了生物标志物的精确检测。在此，我们设计了一种基于脂质体嵌入的 CuAgS 放大的光热传感器，用于在健康监测和即时检测（POCT）中精确测定心肌肌钙蛋白 I（cTnI）。组合式 3D 打印检测设备在近红外（NIR）光的激发下监测和可视化检测系统中的目标信号变化，并用智能手机记录和评估可能的致病信号。值得注意的是，我们通过第一性原理和密度泛函理论（DFT）计算的结构和电荷密度分布，预测了 CuAgS 潜在的高效热转换效率，为新型光热材料的构建提供了理论依据，实验结果也证明了理论预测的正确性。在最佳条件下，光热免疫分析呈现出 0.02-10ng/mL 的动态线性范围，检测限为 11.2pg/mL。这项工作为敏感便携光热生物传感器的发展提供了有前景的理论研究和新的见解。
