University of Veracruz, Health Sciences Institute, Health Sciences Master Program, Mexico; University of Veracruz, Public Health Institute, Mexico.
University of Veracruz, Health Sciences Institute, Health Sciences Doctoral Program, Mexico.
Braz J Infect Dis. 2022 May-Jun;26(3):102357. doi: 10.1016/j.bjid.2022.102357. Epub 2022 May 6.
The purpose of this work was to perform by Whole Genomic Sequencing (WGS) a characterization of tuberculosis isolates circulating in the central region of Veracruz, Mexico, and to determine its geographical distribution. The genome of 25 clinical isolates of tuberculosis patients, recovered from central zone of Veracruz, Mexico, were sequenced and the information obtained was used to characterize lineage, prediction of drug resistance, identification of clonal complexes, and finally correlated with the geolocalization data. Isolates analyzed were included into seven L4 sublineages, most frequent was X3; X1 (4.1.1.3) in 35%. rpoBSer450Leu polymorphism was the most frequently found variant. Sublineage Haarlem (4.1.2) had the widest distribution, found in five municipalities. Of the of two clonal complexes found, the most abundant included eight isolates, with X3/X1 lineage, placed in two municipalities. Combination of WGS and geographic information system was very useful for the identification of sublineages, clonal complexes, and their geographical dispersion with important implications in the epidemiological surveillance and clinical control of TB.
本研究旨在通过全基因组测序（WGS）对墨西哥韦拉克鲁斯州中部流行的结核分枝杆菌分离株进行特征描述，并确定其地理分布。对来自墨西哥韦拉克鲁斯州中部的 25 例结核患者的临床分离株进行基因组测序，利用获得的信息对谱系进行特征描述，预测耐药性，鉴定克隆复合体，最后与地理定位数据相关联。分析的分离株被归入七个 L4 亚谱系，最常见的是 X3；X1（4.1.1.3）占 35%。rpoBSer450Leu 多态性是最常见的变异。哈雷姆（4.1.2）亚谱系分布最广，在五个城市都有发现。在发现的两个克隆复合体中，最丰富的包括八个分离株，属于 X3/X1 谱系，分布在两个城市。WGS 和地理信息系统的结合非常有助于鉴定亚谱系、克隆复合体及其地理分布，这对结核分枝杆菌的流行病学监测和临床控制具有重要意义。
