Endler A T, Young D S, Yanagihara T, Currie R M, Reid J
J Clin Chem Clin Biochem. 1987 Feb;25(2):61-70. doi: 10.1515/cclm.1987.25.2.61.
The protein pattern of cerebrospinal fluid (CSF) of 334 patients with various neurological and systemic diseases was investigated by high resolution two-dimensional electrophoresis (2-DE). 2-DE gels of normal CSF contain proteins which are not detectable in 2-DE gels of serum. Disturbances of the blood-brain or blood-CSF barrier, and degenerative diseases of the brain and malignant diseases produce specific changes on 2-DE gels of the CSF. The appearance of 10 spot areas in the light chain region of 2-DE gels seem to be connected with the diagnosis of multiple sclerosis. The sensitivity and the specificity of these spot areas for the diagnosis of multiple sclerosis are described. Proteins within the ten spot areas are immunoglobulin light chains or substances which cross-react very strongly with light chain antibodies as demonstrated by immunoblotting and immunoabsorption.
采用高分辨率二维电泳（2-DE）技术，对334例患有各种神经和全身性疾病患者的脑脊液（CSF）蛋白质图谱进行了研究。正常脑脊液的2-DE凝胶中含有血清2-DE凝胶中无法检测到的蛋白质。血脑屏障或血脑脊液屏障的破坏，以及脑部退行性疾病和恶性疾病，会使脑脊液的2-DE凝胶产生特定变化。2-DE凝胶轻链区域中10个斑点区域的出现似乎与多发性硬化症的诊断有关。描述了这些斑点区域对多发性硬化症诊断的敏感性和特异性。如免疫印迹和免疫吸收所示，这10个斑点区域内的蛋白质是免疫球蛋白轻链或与轻链抗体发生强烈交叉反应的物质。
