Bracco F, Gallo P, Tavolato B, Battistin L
Neurochem Res. 1985 Sep;10(9):1203-19. doi: 10.1007/BF00964840.
The two-dimensional polyacrylamide gel electrophoresis technique has been adapted for the analysis of human cerebrospinal fluid proteins. Proteins were detected by Coomassie brilliant blue stain and/or by silver stain. Highly reproducible protein patterns were obtained. We analyzed ten normal CSF specimens, thirty pathological CSF specimens and the corresponding sera. We mapped the protein patterns observed by examination of serum/CSF differences and by immunofixation. Preliminary observations on the changes in protein patterns in CSF specimens from patients with neurological disorders are reported.
二维聚丙烯酰胺凝胶电泳技术已被应用于人类脑脊液蛋白质的分析。蛋白质通过考马斯亮蓝染色和/或银染色进行检测。获得了高度可重复的蛋白质图谱。我们分析了10份正常脑脊液标本、30份病理脑脊液标本及相应血清。我们通过检测血清/脑脊液差异和免疫固定来绘制观察到的蛋白质图谱。报告了对神经系统疾病患者脑脊液标本中蛋白质图谱变化的初步观察结果。
