Huang Yu-Ting, Sun Yue, Meng Jiang, Wang Shu-Mei
Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province,Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, National Administration of Traditional Chinese Medicine(TCM), School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2022 Apr;47(7):1765-1775. doi: 10.19540/j.cnki.cjcmm.20220120.201.
Based on the previous research results of our group and literature research, the chemical components, mechanisms, pharmacodynamics, and pharmacokinetics of Zingiberis Rhizoma Carbonisata were summarized to determine the quality markers(Q-markers) of Zingiberis Rhizoma Carbonisata and Zingiberis Rhizoma. Our research group has clarified the differential components of Zingiberis Rhizoma Carbonisata and Zingiberis Rhizoma, the meridian-warming hemostatic effect of Zingiberis Rhizoma Carbonisata, the related targets and pathways of the effect, the endogenous biomarkers of Zingiberis Rhizoma Carbonisata, and the hemodynamic processes of Zingiberis Rhizoma Carbonisata and Zingiberis Rhizoma. Moreover, based on high-performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry(HPLC-DAD-ESIMS), a method for determining the content of Q-mar-kers was established. In conclusion, the study finally determined that gingerone, 6-shogaol, and diacetyl-6-gingerol were the Q-mar-kers of Zingiberis Rhizoma Carbonisata decoction pieces, and 6-gingerol, 8-gingerol, and 10-gingerol were Q-markers of Zingiberis Rhizoma decoction pieces. The result is expected to provide a reference for the establishment of quality standards for Zingiberis Rhizoma Carbonisata decoction pieces and Zingiberis Rhizoma decoction pieces.
基于本课题组前期研究成果及文献研究，对炮姜的化学成分、作用机制、药效学及药代动力学进行总结，以确定炮姜及干姜的质量标志物（Q-标志物）。本课题组已阐明炮姜与干姜的差异成分、炮姜的温经止血作用、该作用的相关靶点及通路、炮姜的内源性生物标志物以及炮姜和干姜的血流动力学过程。此外，基于高效液相色谱-二极管阵列检测器-电喷雾电离质谱联用技术（HPLC-DAD-ESIMS），建立了Q-标志物含量测定方法。综上所述，本研究最终确定姜酮、6-姜烯酚和二乙酰-6-姜酚为炮姜饮片的Q-标志物，6-姜酚、8-姜酚和10-姜酚为干姜饮片的Q-标志物。该结果有望为炮姜饮片和干姜饮片质量标准的建立提供参考。
