Yoshikawa M, Hatakeyama S, Chatani N, Nishino Y, Yamahara J
Kyoto Pharmaceutical University, Japan.
Yakugaku Zasshi. 1993 Apr;113(4):307-15. doi: 10.1248/yakushi1947.113.4_307.
As a continuing study on the evaluation of various Zingiberis Rhizoma and the chemical characterization of the processing, a quantitative method by high performance liquid chromatography (HPLC) for 6, 8, 10-gingerol (1, 2, 3), 6,8-shogaol (4, 5), 6-dehydrogingerdione (6), and galanolactone (7) has been developed. By the use of this HPLC method, the contents of these compound in twenty kinds of Zingiberis Rhizoma [originating in China, Taiwan, Vietnam, and Japan (Shizuoka Prefecture)] and fresh ginger root cultivated in Shizuoka Prefecture were examined. It was found that Japanese Zingiberis Rhizoma and fresh ginger root contained 6-gingerol (1), 6-dehydrogingerdione (6), and galanolactone (7) as major constituents, whereas 7 was not detected in imported Zingiberis Rhizoma and 6 was detected in Vietnamese Zingiberis Rhizoma. Furthermore, the contents of 1 and 7 in fresh ginger root decreased remarkably during the processing procedure for Zingiberis Rhizoma. In addition, anti-ulcer sesquiterpene constituents in seven kinds of Zingiberis Rhizoma were analyzed by means of gas liquid chromatography (GLC).
作为一项关于各种干姜评价及炮制化学特征的持续研究，已开发出一种采用高效液相色谱法（HPLC）测定6,8,10 - 姜酚（1,2,3）、6,8 - 姜烯酚（4,5）、6 - 脱氢姜二酮（6）和姜醇内酯（7）的定量方法。运用该HPLC方法，对二十种干姜（原产于中国、台湾、越南和日本（静冈县））以及静冈县种植的鲜姜根中这些化合物的含量进行了检测。结果发现，日本干姜和鲜姜根以6 - 姜酚（1）、6 - 脱氢姜二酮（6）和姜醇内酯（7）为主要成分，而进口干姜中未检测到7，越南干姜中检测到6。此外，鲜姜根在干姜炮制过程中1和7的含量显著降低。另外，采用气相色谱法（GLC）分析了七种干姜中的抗溃疡倍半萜成分。
