外泌体参与远程缺血再灌注预处理后的肝移植物保护。
Exosome is involved in liver graft protection after remote ischemia reperfusion conditioning.
机构信息
Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou 310022, China; NHC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou 310022, China.
Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
出版信息
Hepatobiliary Pancreat Dis Int. 2023 Oct;22(5):498-503. doi: 10.1016/j.hbpd.2022.04.004. Epub 2022 Apr 25.
BACKGROUND
Remote ischemic perconditioning (RIPerC) has been demonstrated to protect grafts from hepatic ischemia-reperfusion injury (IRI). This study investigated the role of exosomes in RIPerC of liver grafts in rats.
METHODS
Twenty-five rats (including 10 donors) were randomly divided into five groups (n = 5 each group): five rats were used as sham-operated controls (Sham), ten rats were for orthotopic liver transplantation (OLT, 5 donors and 5 recipients) and ten rats were for OLT + RIPerC (5 donors and 5 recipients). Liver architecture and function were evaluated.
RESULTS
Compared to the OLT group, the OLT + RIPerC group exhibited significantly improved liver graft histopathology and liver function (P < 0.05). Furthermore, the number of exosomes and the level of P-Akt were increased in the OLT + RIPerC group.
CONCLUSIONS
RIPerC effectively improves graft architecture and function, and this protective effect may be related to the increased number of exosomes. The upregulation of P-Akt may be involved in underlying mechanisms.
背景
远程缺血预处理（RIPerC）已被证明可保护移植物免受肝缺血再灌注损伤（IRI）。本研究探讨了外泌体在大鼠肝移植物 RIPerC 中的作用。
方法
25 只大鼠（包括 10 只供体）被随机分为五组（每组 n=5）：5 只大鼠作为假手术对照（Sham），10 只大鼠进行原位肝移植（OLT，5 只供体和 5 只受体），10 只大鼠进行 OLT+RIPerC（5 只供体和 5 只受体）。评估肝结构和功能。
结果
与 OLT 组相比，OLT+RIPerC 组肝移植物的组织病理学和肝功能明显改善（P<0.05）。此外，OLT+RIPerC 组的外泌体数量和 P-Akt 水平增加。
结论
RIPerC 可有效改善移植物的结构和功能，这种保护作用可能与外泌体数量的增加有关。P-Akt 的上调可能涉及潜在的机制。
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