Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou 310022, China; NHC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou 310022, China.
Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Hepatobiliary Pancreat Dis Int. 2023 Oct;22(5):498-503. doi: 10.1016/j.hbpd.2022.04.004. Epub 2022 Apr 25.
Remote ischemic perconditioning (RIPerC) has been demonstrated to protect grafts from hepatic ischemia-reperfusion injury (IRI). This study investigated the role of exosomes in RIPerC of liver grafts in rats.
Twenty-five rats (including 10 donors) were randomly divided into five groups (n = 5 each group): five rats were used as sham-operated controls (Sham), ten rats were for orthotopic liver transplantation (OLT, 5 donors and 5 recipients) and ten rats were for OLT + RIPerC (5 donors and 5 recipients). Liver architecture and function were evaluated.
Compared to the OLT group, the OLT + RIPerC group exhibited significantly improved liver graft histopathology and liver function (P < 0.05). Furthermore, the number of exosomes and the level of P-Akt were increased in the OLT + RIPerC group.
RIPerC effectively improves graft architecture and function, and this protective effect may be related to the increased number of exosomes. The upregulation of P-Akt may be involved in underlying mechanisms.
远程缺血预处理（RIPerC）已被证明可保护移植物免受肝缺血再灌注损伤（IRI）。本研究探讨了外泌体在大鼠肝移植物 RIPerC 中的作用。
25 只大鼠（包括 10 只供体）被随机分为五组（每组 n=5）：5 只大鼠作为假手术对照（Sham），10 只大鼠进行原位肝移植（OLT，5 只供体和 5 只受体），10 只大鼠进行 OLT+RIPerC（5 只供体和 5 只受体）。评估肝结构和功能。
与 OLT 组相比，OLT+RIPerC 组肝移植物的组织病理学和肝功能明显改善（P<0.05）。此外，OLT+RIPerC 组的外泌体数量和 P-Akt 水平增加。
RIPerC 可有效改善移植物的结构和功能，这种保护作用可能与外泌体数量的增加有关。P-Akt 的上调可能涉及潜在的机制。
