利用纳米银抑制埃及的万古霉素耐药性。

Inhibition of the Vancomycin Resistance in in Egypt Using Silver Nanoparticles.

机构信息

Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Department of Microbiology and Immunology, Faculty of Pharmacy, Menoufia University, Shebin Elkom, Menoufia, Egypt.

出版信息

Biomed Res Int. 2022 Apr 30;2022:7380147. doi: 10.1155/2022/7380147. eCollection 2022.

is a major human pathogen that is sometimes resistant to vancomycin. In this study, the prevalence of vancomycin-resistant (VRSA) was studied. 100 isolates of . were identified based on biochemical and molecular evidence. The antibiotic susceptibility of the studied isolates was tested against 13 antibiotics by the disc diffusion method that showed 24 vancomycin-resistant isolates. The minimum inhibitory concentrations (MICs) were estimated by the agar dilution method to determine vancomycin intermediate-resistant . (VISA) and VRSA. The resistance gene cluster (, , , and ) was amplified by PCR and then sequenced. Amplification of and genes showed that they are present in 21.4% and 14.3% of VRSA isolates, respectively, whereas none of the studied genes has been detected in VISA strains. A significant antimicrobial effect toward VRSA isolates using silver nanoparticles (AgNPs) synthesized from . and rosemary leaves was recorded. This study confirmed the existence of VRSA strains in Egypt. Furthermore, the use of silver nanoparticles inhibits these vancomycin-resistant . strains .

是一种主要的人类病原体，有时对万古霉素具有耐药性。在本研究中，研究了耐万古霉素的（VRSA）的流行情况。根据生化和分子证据，鉴定了 100 株。采用纸片扩散法对研究分离株进行了 13 种抗生素的药敏试验，显示有 24 株耐万古霉素分离株。采用琼脂稀释法估计最小抑菌浓度（MIC），以确定万古霉素中介耐药（VISA）和 VRSA。通过 PCR 扩增耐药基因簇（、、、和），然后测序。扩增和基因表明，它们分别存在于 21.4%和 14.3%的 VRSA 分离株中，而在 VISA 株中未检测到任何研究基因。从和迷迭香叶合成的银纳米颗粒（AgNPs）对 VRSA 分离株表现出显著的抗菌作用。本研究证实了 VRSA 菌株在埃及的存在。此外，使用银纳米颗粒抑制了这些耐万古霉素的菌株。