Ding Ying, Zhang Du, Wang Min, Zhang Li, Liu Yu, Deng Yinqian, Jiang Dawei, Liu Yuqing, Cao Wei
Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430022, Hubei, China.
Hubei Key Laboratory of Molecular Imaging Wuhan 430022, Hubei, China.
Am J Nucl Med Mol Imaging. 2022 Apr 15;12(2):54-62. eCollection 2022.
Positron emission tomography (PET) can accurately locate and quantify radioactivity over traditional single photon emission computed tomography (SPECT), encouraging its application in kidney function evaluation and glomerular filtration rate (GFR) measurement. Ga-ethylenediamine-tetraacetic acid (Ga-EDTA) is a novel PET tracer for renal scan but a mature GFR calculation method still pending establishment. Herein, we aim to investigate the imaging performance of Ga-EDTA dynamic PET in healthy C57BL/6 mice, establish quantitative methods to calculate GFR, and evaluate its feasibility in mice with kidney dysfunction. Dynamic PET of Ga-EDTA successfully visualized the whole process of tracer elimination. GFR values were measured by the integral method (253.80±40.11 μL/min) and the Patlak Plot method (22.69±9.75 μL/min), while blood clearance rate of the tracer was found at 787.46±70.86 μL/min. The PET-based GFR values correlate well with the GFR (R=0.7468, R=0.8793). The Integral method provides better accuracy than Patlak Plot method. Further application of GFR measurement in kidney-diseased mice proves better performance of the Integral method for defining split renal function.
正电子发射断层扫描（PET）相较于传统的单光子发射计算机断层扫描（SPECT），能够更准确地定位和定量放射性，这促使其在肾功能评估和肾小球滤过率（GFR）测量中得到应用。镓-乙二胺四乙酸（Ga-EDTA）是一种用于肾脏扫描的新型PET示踪剂，但仍有待建立成熟的GFR计算方法。在此，我们旨在研究Ga-EDTA动态PET在健康C57BL/6小鼠中的成像性能，建立计算GFR的定量方法，并评估其在肾功能不全小鼠中的可行性。Ga-EDTA的动态PET成功地可视化了示踪剂消除的全过程。通过积分法（253.80±40.11μL/min）和Patlak Plot法（22.69±9.75μL/min）测量GFR值，同时发现示踪剂的血液清除率为787.46±70.86μL/min。基于PET的GFR值与GFR具有良好的相关性（R=0.7468，R=0.8793）。积分法比Patlak Plot法具有更高的准确性。GFR测量在肾病小鼠中的进一步应用证明，积分法在定义分肾功能方面表现更好。
