Jiang Dawei, Im Hyung-Jun, Boleyn Madeline E, England Christopher G, Ni Dalong, Kang Lei, Engle Jonathan W, Huang Peng, Lan Xiaoli, Cai Weibo
Departments of Radiology and Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Carson International Cancer Center, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518060, China.
Nano Res. 2019 Mar;12(3):637-642. doi: 10.1007/s12274-019-2271-5. Epub 2018 Dec 28.
DNA tetrahedron nanostructure (DTN) is one of the simplest DNA nanostructures and has been successfully applied for biosensing, imaging, and treatment of cancer. To facilitate its biomedical applications and potential clinical translation, fundamental understanding of DTN's transportation among major organs in living organisms becomes increasingly important. Here, we describe the efficient renal clearance of DTN in healthy mice by using positron emission tomography (PET) imaging. The kidney elimination of DTN was later applied for renal function evaluation in murine models of unilateral ureteral obstruction (UUO). We further established a mathematical program of DTN to validate its changes of transportation pattern in healthy and UUO mice. We believe the establishment of pharmacokinetic profiles and mathematical model of DTN may provide insight for future optimization of DNA nanostructures for biomedical applications.
DNA四面体纳米结构（DTN）是最简单的DNA纳米结构之一，已成功应用于生物传感、成像和癌症治疗。为促进其生物医学应用和潜在的临床转化，深入了解DTN在生物体主要器官之间的转运变得越来越重要。在此，我们通过正电子发射断层扫描（PET）成像描述了健康小鼠中DTN的高效肾清除。随后，DTN的肾脏清除被应用于单侧输尿管梗阻（UUO）小鼠模型的肾功能评估。我们进一步建立了DTN的数学程序，以验证其在健康和UUO小鼠中转运模式的变化。我们相信，DTN药代动力学特征和数学模型的建立可能为未来优化用于生物医学应用的DNA纳米结构提供见解。
