Department of Ophthalmology, Boston Children's Hospital, 300 Longwood Ave, Fegan 4, Boston, MA, 02115, USA.
Behavioral Neuroscience, Northeastern University, Boston, MA, USA.
Doc Ophthalmol. 2022 Aug;145(1):1-10. doi: 10.1007/s10633-022-09873-z. Epub 2022 May 10.
To determine the association of the multifocal electroretinographic (mfERG) response amplitude with the volumes of the inner, postreceptor, and photoreceptor retinal layers in the region stimulated by each mfERG element.
Sixteen healthy, young adult control subjects were studied. Each of the 103 hexagonal elements of the standard, scaled mfERG were aligned, where possible, with patches of retina imaged using optical coherence tomography. Stimuli falling on the fovea and on the optic nerve head were excluded. Linear mixed-effects modeling was then used to derive estimated coefficients (voltage/volume) for the mfERG response throughout the full 80 ms standard epoch. The resulting predicted response amplitudes originating in each layer were then compared to pharmacologically "dissected" mfERGs obtained from other studies in monkey eyes.
Across the duration of the response, the amplitude of the modeled contribution from (1) the inner retina was small-to-modest, (2) the postreceptor retina was larger and contained two prominent peaks, and (3) the photoreceptor response was the largest and most closely paralleled the overall (i.e., intact) response, including late-appearing oscillations. The significance of each layer's contribution was greatest when the absolute amplitude of that layer's response was largest. The contribution of the inner retina was maximally significant in the interval between the prominent troughs and peaks of the intact response. The contributions of the postreceptor and photoreceptor responses were maximally significant at the prominent troughs and peaks of the intact response.
The results of the model were in good overall agreement with previous interpretations of the cellular contributions to the mfERG. There was also fair agreement with pharmacologically dissected monkey mfERG responses. Thus, the estimations of the contributions of the retinal layers to the mfERG so produced appeared plausible.
确定多焦视网膜电图（mfERG）反应幅度与受每个 mfERG 元素刺激的内、后受体和光感受器视网膜层体积之间的关系。
研究了 16 名健康的年轻成年对照者。使用光学相干断层扫描对尽可能多的 mfERG 标准、缩放的 103 个六边形元素所对应的视网膜区域进行成像，并对这些元素进行排列。排除落在中央凹和视神经头上的刺激。然后使用线性混合效应模型得出 mfERG 整个 80ms 标准时段的估计系数（电压/体积）。然后将源自每个层的预测响应幅度与从猴眼其他研究中获得的药理学“解剖”mfERG 进行比较。
在整个反应期间，（1）内视网膜的模型贡献幅度较小到中等，（2）后受体视网膜较大，包含两个明显的峰值，（3）光感受器反应最大且最接近整体（即完整）反应，包括后期出现的振荡。当该层响应的绝对幅度最大时，每个层的贡献的重要性最大。内视网膜的贡献在完整响应的明显低谷和峰值之间的间隔内最大。在后受体和光感受器反应的贡献在完整响应的明显低谷和峰值处最大。
该模型的结果与 mfERG 对细胞贡献的先前解释总体上一致。与药理学分离的猴 mfERG 反应也有很好的一致性。因此，产生的这种对 mfERG 中视网膜层贡献的估计似乎是合理的。
