University of Houston, College of Optometry, Houston, Texas, United States.
Invest Ophthalmol Vis Sci. 2014 Jun 26;55(7):4512-24. doi: 10.1167/iovs.14-13937.
We investigated relations between macular retinal ganglion cell plus inner plexiform layer (RGC+IPL) thickness and macular retinal function revealed by multifocal electroretinonography (mfERG) in a nonhuman primate model of experimental glaucoma.
Retinal ganglion cell (RGC) structure and function were followed with spectral-domain optical coherence tomography (SD-OCT) and ERGs in five macaques with unilateral experimental glaucoma. Linear regression was used to study correlations in control (Con) and experimental (Exp) eyes between peripapillary retinal nerve fiber layer (RNFL) thickness, macular RGC+IPL thickness, multifocal photopic negative response (mfPhNR) and high-frequency multifocal oscillatory potentials (mfOP) in slow-sequence mfERG, and low-frequency component (mfLFC) in global-flash mfERG. We used ANOVA and paired t-tests to compare glaucoma-related mfERG changes between superior and inferior hemifields, foveal hexagon, inner three rings, and four quadrants of macula.
Average macular RGC+IPL and temporal RNFL thickness were strongly correlated (r(2) = 0.90, P < 0.001). In hexagon-by-hexagon analysis, all three mfERG measures were correlated (P < 0.001) with RGC+IPL thickness for Con (r(2), 0.33-0.51) and Exp eyes (r(2), 0.17-0.35). The RGC structural and functional metrics decreased as eccentricity increased. The reduction in amplitude of mfERG measures in Exp eyes relative to Con eyes was proportionally greater, in general, than the relative thinning of RGC+IPL at the same location for eyes in which structural loss was not evident, or mild to moderate. Although not statistically significant, percent amplitude reduction of mfERG measures was greatest in the inferior temporal quadrant.
Macular RGC+IPL thickness and mfERG measures of RGC function can be complementary tools in assessing glaucomatous neuropathy.
我们研究了非人类灵长类动物实验性青光眼模型中黄斑视网膜神经节细胞加内丛状层（RGC+IPL）厚度与多焦视网膜电图（mfERG）揭示的黄斑视网膜功能之间的关系。
通过光谱域光学相干断层扫描（SD-OCT）和 ERG 对 5 只单侧实验性青光眼的猕猴进行了视网膜神经节细胞（RGC）结构和功能的随访。在对照（Con）和实验（Exp）眼中，我们使用线性回归研究了视盘周围视网膜神经纤维层（RNFL）厚度、黄斑 RGC+IPL 厚度、多焦明视负反应（mfPhNR）和高频多焦振荡电位（mfOP）之间的相关性在慢序列 mfERG 中，以及全局闪烁 mfERG 中的低频成分（mfLFC）。我们使用 ANOVA 和配对 t 检验比较了上、下半视野、黄斑中心凹六边形、内三环和四个象限之间与青光眼相关的 mfERG 变化。
平均黄斑 RGC+IPL 和颞侧 RNFL 厚度呈强相关（r(2) = 0.90，P < 0.001）。在六边形对六边形分析中，所有三种 mfERG 测量值均与 Con（r(2)，0.33-0.51）和 Exp 眼（r(2)，0.17-0.35）的 RGC+IPL 厚度相关。随着离焦度的增加，RGC 结构和功能指标均降低。与对照眼相比，实验眼 mfERG 测量值的振幅降低幅度通常更大，而在结构损失不明显或轻度至中度的情况下，同一位置的 RGC+IPL 相对变薄。尽管没有统计学意义，但 mfERG 测量值的幅度降低百分比在颞下象限最大。
黄斑 RGC+IPL 厚度和 mfERG 测量的 RGC 功能可以作为评估青光眼神经病变的补充工具。
