ORAOV 1 Detection Made with Metal Organic Frameworks Based on TiCT MXene.

In this work, a two-dimensional (2D) MOF sheet with electrochemiluminescence (ECL) activity is prepared with TiCT MXene as the metal precursor and the meso-tetra(4-carboxyl-phenyl) porphyrin (HTCPP) as the organic ligand. The atomically thin 2D TiCT MXene is utilized as the metal precursor and soft template to produce the MOF with a 2D nanosheet morphology (TiCT-PMOF). TiCT MXene is a kind of strong electron acceptor, which can deprotonate HTCPP due to the high electronegativity and low work function of its terminal atoms. The deprotonated HTCPP continues to bind with Ti atoms to form the 2D MOF sheet. The ECL activity is inherited from HTCPP and stabilized by introducing Ag NPs. Then, we construct an ECL biosensor based on the Ag NPs/TiCT-PMOF to detect the oral cancer overexpressed 1 (ORAOV 1). A bipedal three-dimensional DNA walker strategy is adopted to further improve the biosensor sensitivity. As expected, the biosensor exhibits sterling sensitivity and selectivity. The ECL biosensor responds linearly to ORAOV 1 concentrations in the range of 10 fM-1 nM, and the detection limit is as low as 3.3 fM (S/N = 3). It means that Ag NPs/TiCT-PMOF is a potential material to design and construct the high-performance ECL biosensors.