Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Šlechtitelů 27, 783 71, Olomouc-Holice, Czech Republic.
Univ. Bordeaux, INRAE, UMR 1332 Biologie du Fruit et Pathologie, CS20032, 33882, Villenave d'Ornon Cedex, France.
Arch Virol. 2022 Jul;167(7):1589-1592. doi: 10.1007/s00705-022-05444-4. Epub 2022 May 10.
The genus Cytorhabdovirus includes plant viruses with an unsegmented, single-stranded, negative-sense RNA genome that infect various plant hosts. In this work, we report the detection of a new cytorhabdovirus infecting elderberry (Sambucus nigra L.). Total RNA was purified from infected leaves and, after ribodepletion, sequenced using an Illumina system. The RNA genome of viral isolate B15 is 12,622 nucleotides (nt) long, and that of isolate B42 is 12,621 nt long. A nearly complete sequence (12,592 nt) was also obtained for a third isolate (B160). The RNA genomes of all three isolates showed an organisation typical of cytorhabdoviruses, harbouring all six of the expected genes (3´ N-P-P3-M-G-L 5´), separated by intergenic regions. These isolates were closely related to each other (99.5-99.6% nt sequence identity) and showed the highest overall similarity to trichosanthes associated rhabdovirus 1 (63.5% identity) and Wuhan insect virus 5 (58% identity), and similar results were obtained when comparing individual coding sequences or proteins. Phylogenetic analysis confirmed that this elderberry virus, for which we propose the name "sambucus virus 1" belongs to the genus Cytorhabdovirus and fulfils the criteria to represent a novel species.
该属 Cytorhabdovirus 包括具有非分段的、单链的、负义 RNA 基因组的植物病毒，可感染各种植物宿主。在这项工作中，我们报告了一种新的 Cytorhabdovirus 感染接骨木（Sambucus nigra L.）。从感染的叶片中纯化总 RNA，并在核糖体耗尽后，使用 Illumina 系统进行测序。病毒分离株 B15 的 RNA 基因组长 12622 个核苷酸（nt），分离株 B42 的 RNA 基因组长 12621 nt。还获得了第三个分离株（B160）的几乎完整序列（12592 nt）。所有三个分离株的 RNA 基因组都表现出 Cytorhabdovirus 的典型组织，包含所有六个预期的基因（3´N-P-P3-M-G-L5´），由基因间区隔开。这些分离株彼此之间非常相似（nt 序列同一性为 99.5-99.6%），与 Trichosanthes associated rhabdovirus 1（63.5%同一性）和 Wuhan insect virus 5（58%同一性）的总体相似性最高，当比较单个编码序列或蛋白质时，也得到了相似的结果。系统发育分析证实，这种接骨木病毒，我们提议将其命名为“接骨木病毒 1”，属于 Cytorhabdovirus 属，并符合代表一种新物种的标准。
