Cao Jinying, Xiao Ling, Shi Xiaowen
School of Resource and Environmental Science, Key Laboratory for Biomass Resource Chemistry and Environmental Biotechnology of Hubei Province, Wuhan University Wuhan 430079 China
RSC Adv. 2019 Nov 12;9(63):36858-36866. doi: 10.1039/c9ra07116d. eCollection 2019 Nov 11.
An injectable hydrogel with high adhesion strength, non-toxicity and low cost is highly desired for developing highly efficient hemostasis. In this study, we developed a new type of injectable adhesive drug loaded hydrogel utilizing the formation of Schiff-base linkages based on carboxymethyl chitosan (CMC), gelatin (GEL) and oxidized alginate (OSA). By optimizing the concentration of the biopolymers, the hybrid hydrogel (CMC-GEL/OSA) demonstrated an extremely fast gelation rate (30 s) and adhesive strength of 11 kPa. The freeze-dried hydrogel showed a three-dimensional porous structure. The hydrogel loaded with levofloxacin exhibited good antibacterial properties. Hemostatic performance of the hydrogel was demonstrated in a rat liver injury model. Compared with the untreated wound, the hemostasis time of the hydrogel treated wound was shortened by 84.2% and the blood loss was reduced by 82.2%. Thus, the proposed injectable hydrogel holds great potential applications for hemostasis, drug delivery and in other biomedical fields.
开发高效止血材料非常需要一种具有高粘附强度、无毒且低成本的可注射水凝胶。在本研究中，我们基于羧甲基壳聚糖（CMC）、明胶（GEL）和氧化海藻酸盐（OSA）通过席夫碱键的形成开发了一种新型的载药可注射粘性水凝胶。通过优化生物聚合物的浓度，混合水凝胶（CMC-GEL/OSA）表现出极快的凝胶化速率（30秒）和11 kPa的粘附强度。冻干水凝胶呈现三维多孔结构。负载左氧氟沙星的水凝胶表现出良好的抗菌性能。在大鼠肝损伤模型中证明了该水凝胶的止血性能。与未处理的伤口相比，水凝胶处理的伤口止血时间缩短了84.2%，失血量减少了82.2%。因此，所提出的可注射水凝胶在止血、药物递送和其他生物医学领域具有巨大的潜在应用价值。
