School of Material Science and Engineering, Beijing University of Chemistry Technology, Beijing 100029, China.
Molecules. 2022 Oct 29;27(21):7367. doi: 10.3390/molecules27217367.
Self-healing alginate hydrogels play important roles in the biological field due to their biocompatibility and ability to recover after cracking. One of the primary targets for researchers in this field is to increase the self-healing speed. Sodium alginate was oxidized, generating aldehyde groups on the chains, which were then crosslinked by poly(amino) amine (PAMAM) via Schiff base reaction. The dendritic structure was introduced to the alginate hydrogel in this work, which was supposed to promote intermolecular interactions and accelerate the self-healing process. Results showed that the hydrogel (ADA-PAMAM) formed a gel within 2.5 min with stable rheological properties. Within 25 min, the hydrogel recovered under room temperature. Furthermore, the aldehyde degree of alginate dialdehyde with a different oxidation degree was characterized through gel permeation chromatograph aligned with multi-angle laser light scattering and ultraviolet (UV) absorption. The chemical structure of the hydrogel was characterized through Fourier transform infrared spectroscopy and UV-vis spectra. The SEM and laser scanning confocal microscope (CLSM) presented the antibiotic ability of ADA-PAMAM against both and when incubated with 10 CFU microorganism under room temperature for 2 h. This work presented a strategy to promote the self-healing of hydrogel through forming a dendritic dynamic crosslinking network.
自修复海藻酸钠水凝胶由于其生物相容性和在破裂后能够恢复的能力，在生物领域发挥着重要作用。该领域的研究人员的主要目标之一是提高自修复速度。本工作中通过席夫碱反应将氧化后的海藻酸钠上的醛基与聚（氨基酸）胺（PAMAM）进行交联，在海藻酸钠水凝胶中引入了树枝状结构，这有望促进分子间相互作用并加速自修复过程。结果表明，水凝胶（ADA-PAMAM）在 2.5 分钟内形成凝胶，具有稳定的流变性能。在 25 分钟内，水凝胶在室温下恢复。此外，通过凝胶渗透色谱仪与多角度激光散射和紫外（UV）吸收联用，对不同氧化度的海藻酸钠二醛的醛基程度进行了表征。通过傅里叶变换红外光谱和紫外可见光谱对水凝胶的化学结构进行了表征。SEM 和激光共聚焦显微镜（CLSM）显示，ADA-PAMAM 在室温下与 10 CFU 微生物孵育 2 小时时，对 和 均具有抗生素能力。这项工作提出了一种通过形成树枝状动态交联网络来促进水凝胶自修复的策略。
