Crevillen Agustín G, Mayorga-Martinez Carmen C, Vaghasiya Jayraj V, Pumera Martin
Center for Advanced Functional Nanorobots Department of Inorganic Chemistry University of Chemistry and Technology Prague Technicka 5 Prague 6 166 28 Czech Republic.
Department of Analytical Sciences Faculty of Sciences Universidad Nacional de Educación a Distancia (UNED) Madrid E-28040 Spain.
Adv Mater Technol. 2022 Jun;7(6):2101121. doi: 10.1002/admt.202101121. Epub 2022 Feb 15.
Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.
增材制造技术，也被称为3D打印技术，是一个不断发展的研究领域，其应用范围广泛，从纳米传感器制造到建筑物的3D打印。如今，全球正在应对一场大流行病，需要使用简单的传感系统。在此，将通过基于3D打印技术的芯片实验室设备通过电化学检测对SARS-CoV-2进行快速筛查的优势结合起来。该系统包括一个与由3D打印笔（3D-PP）完全3D打印的电化学池集成的聚二甲基硅氧烷（PDMS）微流体通道。3D-PP基因传感器用靶向SARS-CoV-2 N基因序列的单链DNA（ssDNA）探针进行修饰。传感机制依赖于ssDNA中存在的腺嘌呤在与SARS-CoV-2 RNA接触时的电氧化。ssDNA与靶RNA之间发生杂交，ssDNA从基因传感器表面解吸，导致传感器信号降低。所开发的SARS-CoV-2/3D-PP基因传感器具有高灵敏度和快速响应。
