Tong Yudong, Zhang Yuanhao, Liu Yangyang, Cai Haibo, Zhang Weian, Tan Wen-Song
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology 130 Meilong Road Shanghai 200237 P. R. China
Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology 130 Meilong Road Shanghai 200237 P. R. China
RSC Adv. 2018 Apr 12;8(25):13813-13819. doi: 10.1039/c8ra01584h. eCollection 2018 Apr 11.
Thermosensitive poly(-isopropylacrylamide) (PNIPAM)-based substrates have presented great promise in cell sheet engineering. However, non-functionalized PNIPAM cannot be well applied for cell cultivation, due to the low cell adhesion. Herein, to enhance PNIPAM-based substrates and to promote cell proliferation and detachment, a polyhedral oligomeric silsesquioxane (POSS) nanoscale inorganic enhanced agent has been introduced into PNIPAM matrices to construct POSS-containing hybrid hydrogels. The hydrogels were facilely prepared using POSS as a cross-linker one-pot crosslinking reaction under UV irradiation. The swelling behavior, thermal stability and the mechanical properties of POSS-PNIPAM hybrid hydrogels have been evaluated and they are all dependent on the content of POSS. The experiment confirms that human amniotic mesenchymal stem cells (hAMSCs) exhibit clearly enhanced adhesion and proliferation on the substrates of POSS-PNIPAM hybrid hydrogels in comparison to the pure PNIPAM hydrogel without POSS. Based on the thermal-responsiveness of PNIPAM, the proliferated cells are easily released without damage from the surface of hybrid hydrogels. Therefore, POSS-enhanced PNIPAM hybrid hydrogels provide a unique approach for harvesting anchorage dependent stem cells.
基于热敏性聚（N-异丙基丙烯酰胺）（PNIPAM）的基质在细胞片工程中展现出了巨大的潜力。然而，由于细胞粘附性低，未功能化的PNIPAM不能很好地应用于细胞培养。在此，为了增强基于PNIPAM的基质并促进细胞增殖和脱离，一种多面体低聚倍半硅氧烷（POSS）纳米级无机增强剂被引入到PNIPAM基质中，以构建含POSS的杂化水凝胶。这些水凝胶通过使用POSS作为交联剂，在紫外线照射下进行一锅法交联反应而轻松制备。对POSS-PNIPAM杂化水凝胶的溶胀行为、热稳定性和力学性能进行了评估，它们都取决于POSS的含量。实验证实，与不含POSS的纯PNIPAM水凝胶相比，人羊膜间充质干细胞（hAMSCs）在POSS-PNIPAM杂化水凝胶基质上的粘附和增殖明显增强。基于PNIPAM的热响应性，增殖的细胞很容易从杂化水凝胶表面无损释放。因此，POSS增强的PNIPAM杂化水凝胶为收获贴壁依赖性干细胞提供了一种独特的方法。
