Wu Meiyu, Chen Liang, Li Ruiru, Dan Mo, Liu Haining, Wang Xinsheng, Wu Xiaochun, Liu Ying, Xu Liming, Xie Liming
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, NCNST-NIFDC Joint Laboratory for Measurement and Evaluation of Nanomaterials in Medical Applications, Center for Excellence in Nanoscience, National Center for Nanoscience and Technology No. 11. Beiyitiao Zhongguancun, Haidian District Beijing 100190 P. R. China.
Institute for Medical Devices Control, NCNST-NIFDC Joint Laboratory for Measurement and Evaluation of Nanomaterials in Medical Applications, National Institutes for Food and Drug Control No. 31 Huatuo Road, Biological Medicine Industrial Base, Daxing District Beijing 102629 P. R. China
RSC Adv. 2018 Mar 29;8(22):12260-12268. doi: 10.1039/c8ra00044a. eCollection 2018 Mar 26.
Along with the increasing applications of nanomaterials in medical fields, to know the systemic distribution of nanomaterials in the body through a precise method is required for the biosafety assessment of nanomaterials. In this study, we firstly have established a reliable inductively coupled plasma mass spectrometry (ICP-MS) method for concentration measurement of silver (Ag) and gold (Au) in biological tissues. Then, based on this method, the Ag or Au distribution in rat blood and almost all of the organs were analyzed after an i.v. or s.c. administration of Au@Ag NRs. Both the time-dependent contents of Ag and Au in blood and two pharmacokinetic models confirmed the rapid clearance of Ag from blood. At 24 h after i.v. injection, there was the highest level of Ag in liver, followed by portal nodes, spleen, lung, bone marrow and pancreas. In addition, we also found there were gender-related distributions of Ag and Au in some organs, especially after s.c. injection. Therefore, these more comprehensive and important results would give fundamental information for the biological risk assessment of nanomaterials.
随着纳米材料在医学领域的应用日益增加，通过精确方法了解纳米材料在体内的全身分布对于纳米材料的生物安全性评估至关重要。在本研究中，我们首先建立了一种可靠的电感耦合等离子体质谱（ICP-MS）方法，用于测量生物组织中银（Ag）和金（Au）的浓度。然后，基于该方法，在静脉注射或皮下注射Au@Ag纳米棒后，分析大鼠血液和几乎所有器官中的Ag或Au分布。血液中Ag和Au的时间依赖性含量以及两个药代动力学模型均证实Ag从血液中快速清除。静脉注射后24小时，肝脏中Ag的含量最高，其次是门静脉淋巴结、脾脏、肺、骨髓和胰腺。此外，我们还发现，在某些器官中，尤其是皮下注射后，Ag和Au存在与性别相关的分布。因此，这些更全面和重要的结果将为纳米材料的生物风险评估提供基础信息。
