Wu Hui, Xi Hongru, Lai Furao, Ma Juanjuan, Liu Huifan
College of Food Science and Engineering, South China University of Technology 81 Wu Shan Lu, Tianhe Qu Guangzhou Shi Guangdong Sheng 510640 China
RSC Adv. 2018 Mar 28;8(22):12116-12126. doi: 10.1039/c8ra00142a. eCollection 2018 Mar 26.
Two kinds of flavone extracts were extracted and purified from (LP). High-performance liquid chromatography (HPLC) analysis was used to determinate the flavones in the extracts, and catechin, glycitin, rutin, naringin, and myricetin were identified in the LP leaf extract (LPL-F) while genistin, naringin, and myricetin were found in the stem extract (LPS-F). Specific flavonol compounds mediated the satisfactory scavenging abilities. The flavone leaf extracts performed better than the stem extracts in chemical antioxidative activities but worse in cellular antioxidative capabilities. The chemical and cellular antioxidative activities were not obviously changed by gastrointestinal digestion but slightly changed at the last 2 hours of intestinal digestion because prolonged exposure to alkaline conditions could destroy the structure of flavonoids. Changes in MDA and GSH content, and enzyme activities of SOD and GSH-Px in human erythrocytes during GI digestion indicated the possible intracellular antioxidant-detoxifying mechanisms were through attenuating AAPH-induced oxidative stress by inhibiting ROS generation, in which stem extracts performed the better.
从羽扇豆（LP）中提取并纯化了两种黄酮提取物。采用高效液相色谱（HPLC）分析来测定提取物中的黄酮，在羽扇豆叶提取物（LPL-F）中鉴定出儿茶素、甘草素、芦丁、柚皮苷和杨梅素，而在茎提取物（LPS-F）中发现了染料木苷、柚皮苷和杨梅素。特定的黄酮醇化合物介导了令人满意的清除能力。黄酮叶提取物在化学抗氧化活性方面比茎提取物表现更好，但在细胞抗氧化能力方面则较差。胃肠道消化对化学和细胞抗氧化活性没有明显影响，但在肠道消化的最后2小时略有变化，因为长时间暴露于碱性条件会破坏黄酮类化合物的结构。胃肠道消化过程中人体红细胞中丙二醛（MDA）和谷胱甘肽（GSH）含量的变化以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性表明，可能的细胞内抗氧化解毒机制是通过抑制活性氧（ROS）生成来减轻2,2'-偶氮二异丁基脒二盐酸盐（AAPH）诱导的氧化应激，其中茎提取物表现更佳。
