Wang Qin, Luo Jieying, Liu Huifan, Brennan Charles Stephen, Liu Jianliang, Zou Xiaoyu
College of Light Industry and Food, Zhongkai University of Agriculture and Engineering Guangzhou Guangdong 510225 China
Department of Food, Wine and Molecular Biosciences, University of Lincoln Christchurch New Zealand.
RSC Adv. 2019 May 21;9(28):16007-16017. doi: 10.1039/c9ra02523e. eCollection 2019 May 20.
Pomelo fruitlet is a side-product of pomelo, and this study aimed to extract the antioxidative flavonoid compounds from pomelo fruitlets with high efficiency through ultrasonic-associated microwave methods. Scanning electron microscopy analysis indicated that the spatial structure of the pomelo fruitlet powder was changed; microwaves and ultrasonic waves facilitated the formation of globular and curved surfaces, respectively. Ultrasonic-microwave synergistic pretreatment resulted in significantly higher yield. Each type of flavonoid compound was characterized using PR-LCMS analysis, and naringin with high nutritive value was detected in all groups. After purifying the flavone fractions with AB-8 macroporous resin, naringin, 2''--acetyl-3'--methylrutin, and 5,7,8,3'-tetrahydroxy-3,4'-dimethoxy were identified, which could act as free radical scavengers to protect erythrocytes from AAPH-induced hemolysis. This study strongly improved the effects of ultrasonic-microwave synergetic methods on the high utilization of pomelo fruitlets, especially in terms of flavonoid extraction and bioavailability.
柚子幼果是柚子的副产品，本研究旨在通过超声联合微波法高效提取柚子幼果中的抗氧化类黄酮化合物。扫描电子显微镜分析表明，柚子幼果粉末的空间结构发生了变化；微波和超声波分别促进了球状和曲面的形成。超声-微波协同预处理的产率显著更高。使用PR-LCMS分析对每种类黄酮化合物进行了表征，所有组中均检测到了具有高营养价值的柚皮苷。用AB-8大孔树脂纯化黄酮馏分后，鉴定出了柚皮苷、2''-乙酰基-3'-甲基芦丁和5,7,8,3'-四羟基-3,4'-二甲氧基，它们可作为自由基清除剂来保护红细胞免受AAPH诱导的溶血。本研究极大地提高了超声-微波协同方法对柚子幼果高利用率的效果，尤其是在类黄酮提取和生物利用度方面。
