Long Xiufeng, Tian Jiewei, Liao Xuepin, Tian Yongqiang
Department of Biomass and Leather Engineering, Sichuan University Chengdu 610065 PR China
National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University Chengdu 610065 PR China.
RSC Adv. 2018 Aug 2;8(48):27525-27536. doi: 10.1039/c8ra05472j. eCollection 2018 Jul 30.
Genomic sequence, transcriptomic, metabolomic and fatty acid analyses of strain HNA-14 were performed to understand the mechanism of salt tolerance for long-term survival. The results indicated that strain HNA-14 has different osmotic resistance mechanisms for long-term survival and short-term salt stress. The cells mainly synthesized compatible solutes to resist osmotic pressure when cultured under nutrient deficient conditions, while they can slow down the synthesis rate and uptake from the environment when cultured under a nutritionally rich environment. Also, the amounts of branched and unsaturated fatty acids in the cell membrane are maintained to a high degree (>50%) to maintain the fluidity of the cell membrane; when the cells are cultured in a high osmotic environment for long-term survival, they may increase the content of branched fatty acids and phosphoric fatty acids to increase the fluidity of the cell membrane to resist the high osmotic pressure.
对菌株HNA-14进行了基因组序列、转录组学、代谢组学和脂肪酸分析，以了解其长期存活的耐盐机制。结果表明，菌株HNA-14在长期存活和短期盐胁迫下具有不同的抗渗透机制。当在营养缺乏条件下培养时，细胞主要合成相容性溶质以抵抗渗透压，而在营养丰富的环境中培养时，它们可以减缓合成速率并从环境中摄取。此外，细胞膜中支链脂肪酸和不饱和脂肪酸的含量保持在较高水平（>50%）以维持细胞膜的流动性；当细胞在高渗环境中培养以长期存活时，它们可能会增加支链脂肪酸和磷酸脂肪酸的含量，以增加细胞膜的流动性来抵抗高渗透压。
