Sheng Tao, Zhao Lei, Gao Lingfang, Liu Wenzong, Wu Guofeng, Wu Jieting, Wang Aijie
State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology Harbin 150090 China
College of Environmental and Chemical Engineering, Heilongjiang Institute of Science and Technology Harbin China.
RSC Adv. 2018 Jun 22;8(41):22924-22930. doi: 10.1039/c8ra03361g. eCollection 2018 Jun 21.
An edible fungal pretreatment of rice straw was proposed for enhanced hydrogen production while reducing the chemical cost for traditional biological hydrogen production from lignocellulose. In this research, rice straw was pretreated by edible fungus J2 at room temperature under static conditions for 15 d at first. The highest hydrogen yield of 5.71 mmol g-pretreated rice straw was obtained, 74% higher than the counterpart without pretreatment. Chemical composition analysis demonstrated that lignin removal was up to 22.4% with a little cellulose and hemicellulose loss of 13.3% and 17.1%, respectively, which is in favor of hydrogen production. Additionally, microscopic structure observation combined with FT-IR and XRD analysis illustrated the structural disruption of pretreated rice straw, and the crystalline index of rice straw can be decreased by 46.2% after pretreatment, which might account for the hydrogen production enhancement. The results also indicated that the hydrogen yield from pretreated rice straw was not affected without the addition of yeast extract and vitamins to the culture medium, which is substantial evidence that edible fungal pretreated rice straw could provide prerequisite nutrients for hydrogen-producing bacteria. Overall, edible fungal pretreatment has great potential under the mild conditions for high hydrogen yields and thus leads to a new direction to realize a highly efficient and economically competitive biological hydrogen production process from lignocellulosic biomass.
提出了一种用于稻草的食用菌预处理方法，以提高产氢量，同时降低传统木质纤维素生物制氢的化学成本。在本研究中，首先在室温静态条件下，用食用菌J2对稻草进行预处理15天。获得了最高产氢量为5.71 mmol g-预处理稻草，比未预处理的稻草高出74%。化学成分分析表明，木质素去除率高达22.4%，纤维素和半纤维素分别有少量损失，为13.3%和17.1%，这有利于产氢。此外，微观结构观察结合傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）分析表明预处理稻草的结构被破坏，预处理后稻草的结晶指数可降低46.2%，这可能是产氢量增加的原因。结果还表明，在培养基中不添加酵母提取物和维生素的情况下，预处理稻草的产氢量不受影响，这充分证明了食用菌预处理的稻草可为产氢细菌提供必要的营养物质。总体而言，食用菌预处理在温和条件下具有实现高产氢量的巨大潜力，从而为实现从木质纤维素生物质中高效且具有经济竞争力的生物制氢过程开辟了新方向。
