Xu Huihui, Cai Qiyong, Nie Qiuyu, Qiao Zhun, Liu Song, Li Zhaohui
Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University Changsha 410082 P. R. China
College of Chemistry and Molecular Engineering, Zhengzhou University Zhengzhou 450001 P. R. China
RSC Adv. 2018 Jun 29;8(42):23720-23726. doi: 10.1039/c8ra03528h. eCollection 2018 Jun 27.
Ascorbic acid (AA) as an essential vitamin in the human body participates in various physiological reactions and plays a key role in many biochemical processes. Therefore, it is of vital importance to monitor and quantify AA in commercial tablets, beverages and food. In this work, a rapid and ultrasensitive chemiluminescence (CL) system for the detection of AA was developed, in which ultrathin cobalt oxyhydroxide (CoOOH) nanosheets were applied in the conventional luminol-HO CL system. The results showed that ultrathin CoOOH nanosheets as a catalyzer remarkably improved the CL intensity of the CoOOH-luminol-HO system, up to about 1400-fold. Under the optimized conditions, the CL inhibition efficiencies increase linearly with the concentrations of AA in the range of 1-500 pmol L, and the limit of detection was 39 fmol L. Moreover, the proposed CL system was successfully applied in the determination of AA in medicinal tablets with satisfactory results.
抗坏血酸（AA）作为人体必需的维生素，参与各种生理反应，并在许多生化过程中起关键作用。因此，监测和定量商业片剂、饮料和食品中的AA至关重要。在这项工作中，开发了一种用于检测AA的快速超灵敏化学发光（CL）系统，其中超薄氢氧化钴（CoOOH）纳米片应用于传统的鲁米诺-HO CL系统。结果表明，超薄CoOOH纳米片作为催化剂显著提高了CoOOH-鲁米诺-HO系统的CL强度，提高了约1400倍。在优化条件下，CL抑制效率在1-500 pmol L范围内随AA浓度呈线性增加，检测限为39 fmol L。此外，所提出的CL系统成功应用于药片中AA的测定，结果令人满意。
