Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, P. R. China.
Analyst. 2021 Mar 21;146(6):1981-1985. doi: 10.1039/d0an02280b. Epub 2021 Jan 27.
We find that luminol can react with artemisinin (ART) to produce chemiluminescence (CL) in the absence of a catalyst and ascorbic acid (AA) can quench luminol-ART CL. Based on its efficient inhibition effect on luminol-ART CL, a new AA detection method is established. The calibration curve for the determination of AA is in the linear range of 5 × 10 M to 1 × 10 M with a detection limit of 50 nM, which is more sensitive than many other reported methods. This CL approach was utilized to detect AA in vitamin C tablets by applying the standard addition method, and the recoveries of 104.0%, 96.8% and 103.4% were obtained, respectively, at concentrations of 1 μM, 5 μM and 10 μM with a RSD value of less than 3.6%. This developed method for AA assay is distinguished by its fastness, reproducibility, easy operation and good selectivity.
我们发现鲁米诺在没有催化剂和抗坏血酸（AA）的情况下可以与青蒿素（ART）反应产生化学发光（CL），而抗坏血酸（AA）可以猝灭鲁米诺-ART CL。基于其对鲁米诺-ART CL 的有效抑制作用，建立了一种新的 AA 检测方法。AA 的测定校准曲线在 5×10-7 M 至 1×10-5 M 范围内呈线性，检测限为 50 nM，比许多其他报道的方法更灵敏。该 CL 方法通过应用标准加入法用于检测维生素 C 片中的 AA，在浓度为 1 μM、5 μM 和 10 μM 时，回收率分别为 104.0%、96.8%和 103.4%，RSD 值小于 3.6%。该用于 AA 测定的方法具有快速、重现性好、操作简单和选择性好的特点。
