Zhou Hong, Qu Zhuo, Zhang Jingze, Jiang Bingjie, Liu Changxiao, Gao Wenyuan
Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University Weijin Road Tianjin 300072 China pharmgao@tju,edu.cn +86-22-87401895 +86-22-87401895.
No. 6 Traditional Chinese Medicine Factory, Tianjin Zhongxin Pharmaceutical Group Corporation Ltd. Tianjin 300401 China.
RSC Adv. 2018 Mar 13;8(19):10163-10171. doi: 10.1039/c7ra13726e.
The Shunaoxin dropping pill (SNX) is derived from a traditional recipe. It has been used to treat cerebrovascular diseases in China since 2005 (approval number Z20050041). In this study, we used an HO-induced PC12 cell oxidative damage model and an d-gal-induced mouse memory impairment model to investigate whether SNX had neuroprotective effects. , prior to exposure to 100 μM HO for 2 h, PC12 cells were pre-treated with SNX 50 μg mL for 24 h. Hoechst 33258 staining was used to confirm the effect of SNX on apoptosis in the PC12 cells. Our results demonstrate that HO suppresses the proliferation of PC12 cells and induces cell death. Pretreatment with SNX attenuates HO-induced apoptosis in PC12 cells. , d-gal was administered (100 mg kg, subcutaneously (s.c.)) once daily for 8 weeks to induce memory deficit and neurotoxicity in the brain of an aging mouse. Then, SNX (320 mg kg) was simultaneously administered orally. The present study demonstrates that SNX can alleviate aging in the mouse brain induced by d-gal improving behavioral performance, alleviating oxidative stress, inhibiting neuroinflammation, and reducing brain cell damage in the hippocampus. Overall, these data clearly demonstrate the neuroprotective effect of SNX from the and results. SNX may be considered a novel agent for easing aging and/or age-related neurodegenerative diseases.
舒脑欣滴丸（SNX）源自传统配方。自2005年起（批准文号Z20050041），它在中国被用于治疗脑血管疾病。在本研究中，我们使用过氧化氢（HO）诱导的PC12细胞氧化损伤模型和D-半乳糖（d-gal）诱导的小鼠记忆损伤模型，来研究SNX是否具有神经保护作用。在暴露于100μM HO 2小时之前，PC12细胞用50μg/mL的SNX预处理24小时。使用Hoechst 33258染色来确认SNX对PC12细胞凋亡的影响。我们的结果表明，HO抑制PC12细胞的增殖并诱导细胞死亡。SNX预处理可减轻HO诱导的PC12细胞凋亡。此外，每天一次皮下注射（s.c.）100mg/kg的d-gal，持续8周，以诱导衰老小鼠大脑中的记忆缺陷和神经毒性。然后，同时口服给予SNX（320mg/kg）。本研究表明，SNX可以减轻d-gal诱导的小鼠大脑衰老，改善行为表现，减轻氧化应激，抑制神经炎症，并减少海马体中的脑细胞损伤。总体而言，这些数据从上述结果清楚地证明了SNX的神经保护作用。SNX可能被认为是一种缓解衰老和/或与年龄相关的神经退行性疾病的新型药物。
