Bhowmick Sirsendu, Thanusha A V, Kumar Arun, Scharnweber Dieter, Rother Sandra, Koul Veena
Max-Bergmann Center of Biomaterials Dresden, Technische Universität Dresden Budapester Straße 27 01069 Dresden Germany.
Centre for Biomedical Engineering, Indian Institute of Technology Delhi Hauz Khas New Delhi 110016 India
RSC Adv. 2018 May 3;8(30):16420-16432. doi: 10.1039/c8ra01489b.
The aim of this study was to investigate the efficacy of a skin substitute composed of mPEG-PCL-grafted-gelatin (Bio-Syn)/hyaluronan/chondroitin sulfate/sericin and to study its biocompatibility with human fibroblasts, human keratinocytes and hMSCs in terms of cellular adhesion and proliferation (∼5-6 fold). mPEG-PCL was grafted into a gelatin backbone a Michael addition reaction to prepare Bio-Syn and it was characterized using ATR-FTIR, H NMR and TNBS assay. Additionally, keratinocyte-hMSC contact co-culture studies showed that Bio-Syn composite scaffolds loaded with sericin promote hMSCs' epithelial differentiation with regard to qRT-PCR gene expression (ΔNp63α and keratin 14) and expression of various epithelial markers (Pan-cytokeratin, ΔNp63α and keratin 14). efficacy studies on a 2 degree burn wound model in Wistar rats showed an improved rate of wound contraction, histology (H&E and Van Gieson's staining) and pro-healing marker (hexosamine, hydroxyproline, ) expression in granular tissue compared to using the commercial dressing Neuskin™ and a cotton gauze control.
本研究的目的是研究由甲氧基聚乙二醇-聚己内酯接枝明胶（Bio-Syn）/透明质酸/硫酸软骨素/丝胶蛋白组成的皮肤替代物的疗效，并从细胞黏附和增殖（约5-6倍）方面研究其与人成纤维细胞、人角质形成细胞和人间充质干细胞的生物相容性。通过迈克尔加成反应将甲氧基聚乙二醇-聚己内酯接枝到明胶主链上制备Bio-Syn，并使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、核磁共振氢谱（H NMR）和三硝基苯磺酸（TNBS）测定对其进行表征。此外，角质形成细胞-人间充质干细胞接触共培养研究表明，负载丝胶蛋白的Bio-Syn复合支架在定量逆转录聚合酶链反应（qRT-PCR）基因表达（ΔNp63α和角蛋白14）以及各种上皮标志物（泛细胞角蛋白、ΔNp63α和角蛋白14）表达方面促进人间充质干细胞的上皮分化。在Wistar大鼠二度烧伤创面模型上的疗效研究表明，与使用商业敷料Neuskin™和棉纱布对照相比，创面收缩率、组织学（苏木精-伊红染色和范吉森染色）以及颗粒组织中促愈合标志物（氨基己糖、羟脯氨酸）的表达均有所改善。
