Yoshikawa H, Yoshinaga M, Tamiya E
Department of Applied Physics, Osaka University Suita Osaka 565-0871 Japan
RSC Adv. 2018 Apr 18;8(26):14510-14514. doi: 10.1039/c8ra01149d. eCollection 2018 Apr 17.
We evaluated optical pickup ELISA with an original microfluidic disk that contains eight radially arranged channels, which enable semi-automatic sample loading and washing. This disk-shaped chip composed of acrylic plates was fabricated by CO laser machining and capture antibodies were immobilized in the channels. After the immunoreaction with antigens and enzyme-linked secondary antibodies, an enzyme-catalyzed nanoaggregation of -phenylenediamine was detected by measuring the reflectivity change of a laser beam focused in the channel. The assay of C-reactive protein (CRP) was successfully performed in a short amount of time (approximately 20 min from CRP loading). The limit of detection was determined to be 2 ng mL, which is more sensitive as compared with conventional ELISA using microplates.
我们使用了一种原始的微流控盘对光学读取酶联免疫吸附测定（ELISA）进行了评估，该微流控盘包含八个径向排列的通道，能够实现半自动进样和洗涤。这种由丙烯酸板组成的盘状芯片是通过CO激光加工制造的，捕获抗体固定在通道中。在与抗原和酶联二抗进行免疫反应后，通过测量聚焦在通道中的激光束的反射率变化来检测对苯二胺的酶催化纳米聚集。C反应蛋白（CRP）的检测在短时间内（从加载CRP开始约20分钟）成功完成。检测限确定为2 ng/mL，与使用微孔板的传统ELISA相比更灵敏。
