Carnerero Jose M, Jimenez-Ruiz Aila, Castillo Paula M, Prado-Gotor Rafael
Physical Chemistry. Faculty of Chemistry, University of Seville, C/Profesor Garcia Gonzalez, s/n, 41012, Seville, Spain.
Chemphyschem. 2017 Jan 4;18(1):17-33. doi: 10.1002/cphc.201601077. Epub 2016 Oct 27.
The interactions of DNA, whether long, hundred base pair chains or short-chained oligonucleotides, with ligands play a key role in the field of structural biology. Its biological activity not only depends on the thermodynamic properties of DNA-ligand complexes, but can and often is conditioned by the formation kinetics of those complexes. On the other hand, gold nanoparticles have long been known to present excellent biocompatibility with biomolecules and are themselves remarkable for their structural, electronic, magnetic, optical and catalytic properties, radically different from those of their counterpart bulk materials, and which make them an important asset in multiple applications. Therefore, thermodynamic and kinetic studies of the interactions of DNA with nanoparticles acting as small ligands are key for a better understanding of those interactions to allow for their control and modulation and for the opening of new venues of research in nanomedicine, analytic and biologic fields. The interactions of gold nanoparticles with both DNA polymers and their smaller subunits; special focus is placed on those interactions taking place with nonfunctionalized gold nanoparticles are reviewed in the present work.
DNA（无论是长的、数百个碱基对的链还是短链寡核苷酸）与配体的相互作用在结构生物学领域起着关键作用。其生物活性不仅取决于DNA-配体复合物的热力学性质，而且能够且常常受到这些复合物形成动力学的制约。另一方面，长期以来人们都知道金纳米颗粒与生物分子具有出色的生物相容性，并且其本身在结构、电子、磁性、光学和催化性质方面表现卓越，与它们的块状材料对应物截然不同，这使其成为多种应用中的重要资产。因此，对作为小配体的纳米颗粒与DNA相互作用进行热力学和动力学研究，对于更好地理解这些相互作用以实现对其控制和调节，以及在纳米医学、分析和生物学领域开辟新的研究方向至关重要。本文综述了金纳米颗粒与DNA聚合物及其较小亚基的相互作用；特别关注了与未功能化金纳米颗粒发生的那些相互作用。
