Zhang Dan, Gao Yun-Ling, Jiang Sheng, Chen Yiwen, Zhang Yi, Pan Zheng
College of Traditional Chinese Medicine, Chongqing Medical University Chongqing China
School of Bio-information, Chongqing University of Post and Telecommunications Chongqing China.
RSC Adv. 2018 Jan 10;8(5):2459-2468. doi: 10.1039/c7ra10143k. eCollection 2018 Jan 9.
() is a Tibetan medicinal herb used for centuries that contains iridoid glycosides (IGs), which are pharmacologically active ingredients and can be used for quality control. The IG profiles of the underground and aerial parts of the plant were determined by UPLC-TOF-MS to evaluate the similarity and variability of the different herbal parts listed in the Chinese Pharmacopoeia. Twenty-six IGs were detected in the total ion current (TIC) profile of , and twenty-two of these were identified by comparing the retention times and mass spectra of the compounds to those of authentic standards. Among these compounds, five IGs with the same molecular formula (CHO) were identified for the first time by mass spectrometry based on their different hydroxyl group-substituted positions. The aerial part has a similar chemical profile to that of the roots. The difference between the two parts was determined by multivariate statistical analysis of the UPLC-TOF-MS data of 24 specimens. Sesamoside was explored as the most characteristic marker to distinguish the two parts of . To further estimate the distinction between the two parts, the content of total IGs and the antioxidant capacity were investigated in samples from different locations. The aerial parts showed a high content of total IGs and high antioxidant capacity, although not higher than those of the roots. The results also suggest the dosage should be increased when the aerial parts are used as crude medicinal materials instead of the underground parts.
（）是一种已使用数百年的藏药，含有环烯醚萜苷（IGs），这些是药理活性成分，可用于质量控制。通过超高效液相色谱-飞行时间质谱（UPLC-TOF-MS）测定该植物地下部分和地上部分的IG图谱，以评估《中国药典》中列出的不同草药部位的相似性和变异性。在（）的总离子流（TIC）图谱中检测到26种IGs，其中22种通过将化合物的保留时间和质谱与标准品进行比较而得以鉴定。在这些化合物中，基于其不同的羟基取代位置，通过质谱首次鉴定出5种具有相同分子式（CHO）的IGs。地上部分与根部具有相似的化学特征。通过对24个样本的UPLC-TOF-MS数据进行多元统计分析来确定这两个部位之间的差异。芝麻苷被确定为区分（）两个部位的最具特征性的标志物。为了进一步评估这两个部位之间的差异，对来自不同产地的样本中总IGs的含量和抗氧化能力进行了研究。地上部分显示出较高的总IGs含量和较高的抗氧化能力，尽管不高于根部。结果还表明，当使用地上部分而非地下部分作为原料药时，应增加用药剂量。
