Raksawong Phannika, Chullasat Kochaporn, Nurerk Piyaluk, Kanatharana Proespichaya, Davis Frank, Bunkoed Opas
Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Anal Bioanal Chem. 2017 Aug;409(20):4697-4707. doi: 10.1007/s00216-017-0466-8. Epub 2017 Jul 6.
A hybrid molecularly imprinted polymer (MIP)-coated quantum dot (QD) nanocomposite was synthesized and applied as a fluorescence probe for the highly sensitive and selective determination of salbutamol. The hybrid MIP-coated QD nanocomposite was synthesized via a copolymerization process in the presence of thioglycolic acid capped CdTe QDs with salbutamol as a template, 3-aminopropyltriethoxysilane as the functional monomer, and tetraethyl orthosilicate as a cross-linker. The optimum molar ratio of template, monomer, and cross-linker was 1:6:20. The fluorescence intensity of the hybrid MIP-coated QDs was efficiently quenched after salbutamol rebound to the recognition sites, as a result of charge transfer from QDs to salbutamol. The synthesized hybrid MIP-coated QD nanocomposite showed high sensitivity and good selectivity toward salbutamol. Under the optimal recognition conditions, the fluorescence intensity was quenched linearly with increasing concentration of salbutamol in the range from 0.10 to 25.0 μg L, with a detection limit of 0.034 μg L. The hybrid optosensor developed was successfully applied in the determination of salbutamol in animal feeds and meat samples. Satisfactory recoveries were obtained in the range from 85% to 98%, with a standard deviation of less than 8%. Furthermore, the accuracy of the hybrid MIP-coated QD nanocomposite was investigated by comparison with a conventional high-performance liquid chromatography method, with the results obtained with two methods agreeing well with each other. The advantages of this sensing method are simplicity, rapidity, cost-effectiveness, high sensitivity, and good selectivity. Graphical Abstract The synthesis of hybrid MIP-coated QDs nanocomposite.
合成了一种杂化分子印迹聚合物（MIP）包覆量子点（QD）的纳米复合材料，并将其用作荧光探针，用于高灵敏度和选择性地测定沙丁胺醇。通过在巯基乙酸包覆的CdTe量子点存在下，以沙丁胺醇为模板、3-氨丙基三乙氧基硅烷为功能单体、正硅酸四乙酯为交联剂的共聚过程，合成了杂化MIP包覆的QD纳米复合材料。模板、单体和交联剂的最佳摩尔比为1:6:20。由于电荷从量子点转移到沙丁胺醇，沙丁胺醇反弹到识别位点后，杂化MIP包覆量子点的荧光强度被有效猝灭。合成的杂化MIP包覆QD纳米复合材料对沙丁胺醇表现出高灵敏度和良好的选择性。在最佳识别条件下，荧光强度随沙丁胺醇浓度在0.10至25.0 μg/L范围内增加而呈线性猝灭，检测限为0.034 μg/L。所开发的杂化光传感器成功应用于动物饲料和肉类样品中沙丁胺醇的测定。回收率在85%至98%范围内，标准偏差小于8%，结果令人满意。此外，通过与传统高效液相色谱法比较，研究了杂化MIP包覆QD纳米复合材料的准确性，两种方法得到的结果吻合良好。这种传感方法的优点是简单、快速、成本效益高、灵敏度高和选择性好。图形摘要：杂化MIP包覆量子点纳米复合材料的合成。
