基于磁性介孔硅微球的新型分子印迹聚合物用于选择性和高效测定丁香中原儿茶酸。
Novel molecular imprinted polymers over magnetic mesoporous silica microspheres for selective and efficient determination of protocatechuic acid in Syzygium aromaticum.
机构信息
College of Sciences, Central South University of Forestry and Technology, Changsha 410004, China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
出版信息
Food Chem. 2015 Jul 1;178:18-25. doi: 10.1016/j.foodchem.2015.01.069. Epub 2015 Jan 21.
Improving sites accessibility can increase the binding efficiency of molecular imprinted polymers (MIPs). In this work, we firstly synthesized MIPs over magnetic mesoporous silica microspheres (Fe3O4@mSiO2@MIPs) for the selective recognition of protocatechuic acid (PCA). The resulting Fe3O4@mSiO2@MIPs were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FT-IR), thermo-gravimetric analysis (TGA), Brunauer-Emmett-Teller (BET), and vibration sample magnetometer (VSM), and evaluated by adsorption isotherms/kinetics and competitive adsorption. The maximum adsorption capacity of PCA on Fe3O4@mSiO2@MIPs was 17.2mg/g (2.3 times that on Fe3O4@SiO2@MIPs). In addition, Fe3O4@mSiO2@MIPs showed a short equilibrium time (140min), rapid magnetic separation (5s) and high stability (retained 94.4% after six cycles). Subsequently, Fe3O4@mSiO2@MIPs were successfully applied for the selective and efficient determination of PCA (29.3μg/g) from Syzygium aromaticum. Conclusively, we combined three advantages into Fe3O4@mSiO2@MIPs, namely, Fe3O4 core for quick separation, mSiO2 layer for enough accessible sites, and surface imprinting MIPs for fast binding and excellent selectivity, to extract PCA from complex systems.
提高分子印迹聚合物（MIPs）的结合效率可以改善其对目标物的识别性能。本工作首先通过一步法合成了磁性介孔硅球表面分子印迹聚合物（Fe3O4@mSiO2@MIPs），用于原儿茶酸（PCA）的选择性识别。采用透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、BET 比表面积测试和振动样品磁强计（VSM）对其进行了结构和性能表征，并通过吸附等温线/动力学和竞争吸附实验对其吸附性能进行了评价。结果表明，Fe3O4@mSiO2@MIPs 对 PCA 的最大吸附量为 17.2mg/g（是 Fe3O4@SiO2@MIPs 的 2.3 倍）。此外，Fe3O4@mSiO2@MIPs 还具有较短的平衡时间（140min）、快速的磁分离（5s）和较高的稳定性（六次循环后仍保留 94.4%的吸附量）。随后，将 Fe3O4@mSiO2@MIPs 成功应用于从丁香中选择性和高效地测定 PCA（29.3μg/g）。总之，我们将 Fe3O4 核用于快速分离、mSiO2 层用于提供足够的可及位点、表面印迹 MIPs 用于快速结合和优异的选择性这三种优势结合到 Fe3O4@mSiO2@MIPs 中，用于从复杂体系中提取 PCA。
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