根据欧洲标准 PN-EN 17126:2019-01，过氧乙酸消毒剂对枯草芽孢杆菌和蜡样芽孢杆菌孢子的杀菌活性。

The sporicidal activity of a disinfectant with peracetic acid against the spores of Bacillus subtilis and Bacillus cereus according to the european standard PN-EN 17126: 2019-01.

机构信息

National Institute of Public Health NIH - National Research Institute, Department of Bacteriology and Biocontamination Control.

出版信息

Przegl Epidemiol. 2021;75(4):524-536. doi: 10.32394/pe.75.49.

DOI:10.32394/pe.75.49

PMID:35543455

Abstract

INTRODUCTION

The assessment of the sporicidal effectiveness of disinfectants is important from the point of view of the prevention of nosocomial infections and spore contamination of clinical samples, medical equipment and materials used in patient care. The rods of Bacillus spp. cause infections of the digestive system, bloodstream and, less often, respiratory tract. Cases were diagnosed in immunocompromised patients, malignant neoplasms and in neonatal wards. The source of the infection was hospital linen, reusable towels, catheters or milk from the human milk bank.

AIM OF THE STUDY

Determination of the minimal sporicidal parameters of a disinfectant containing peracetic acid.

MATERIAL AND METHODS

The sporicidal activity of a disinfectant containing peracetic acid against Bacillus subtilis and Bacillus cereus spore suspensions was tested in a defined concentration range during a contact time of 15 minutes, in the presence of various interfering substances (clean and dirty conditions) according to the European Standard PN-EN 17126: 2019-01.

RESULTS

The disinfecting preparation containing peracetic acid showed sporicidal activity against Bacillus subtilis at a concentration of 1% for 15 minutes under clean and dirty conditions and at concentrations of 0.5%, 1.00% and 1.25% against the Bacillus cereus spores during the same contact time but only under dirty conditions. The preparation showed no sporicidal activity against Bacillus cereus at concentrations of 2%, 3%, 4% and 5% during a contact time of 15 minutes under both dirty and clean conditions.

CONCLUSIONS

In areas where there is a risk of infecting a patient or contaminating clinical specimens, materials and equipment with spores of Bacillus spp., it is necessary to use disinfectants with sporicidal activity confirmed according to the PN-EN 17126: 2019-01 standard. The sporicidal activity of disinfectants containing peracetic acid may depend on the method of preparing the solutions, their concentration, pH, temperature and the contamination degree of the disinfected surface.

摘要

简介

从预防医院感染和临床样本、医疗设备和材料污染的角度来看，评估消毒剂的杀菌效果非常重要。芽孢杆菌属的杆菌会引起消化系统、血液系统感染，较少见的还有呼吸道感染。这些病例发生在免疫功能低下的患者、恶性肿瘤患者和新生儿病房中。感染源是医院的亚麻布、可重复使用的毛巾、导管或人乳库中的牛奶。

研究目的

确定含有过氧乙酸的消毒剂的最低杀菌参数。

材料和方法

根据欧洲标准 PN-EN 17126:2019-01，在 15 分钟接触时间内，在存在各种干扰物质（清洁和污染条件）的情况下，测试了一种含有过氧乙酸的消毒剂对枯草芽孢杆菌和蜡状芽孢杆菌芽孢悬液的杀菌活性，浓度范围在规定范围内。

结果

在清洁和污染条件下，含过氧乙酸的消毒剂在 1%浓度下对枯草芽孢杆菌具有杀菌活性，在 15 分钟接触时间内对蜡状芽孢杆菌孢子的浓度为 0.5%、1.00%和 1.25%，但仅在污染条件下有效。该制剂在 15 分钟接触时间内，在清洁和污染条件下，浓度为 2%、3%、4%和 5%时，对蜡状芽孢杆菌均无杀菌活性。